FORMULATION AND ANTIMALARIAL ACTIVITY TEST OF CHITOSAN BASED NANOPARTICLES CONTAINING ANTISENSE OLIGODEOXYNUCLEOTIDE TARGETED GENES eba-175 AND dhs
Malaria is one of the infectious diseases caused by Plasmodium parasites. Plasmodium falciparum is the most serious form of the disease causes. The main problem of malarial treated currently are multiple drug resistance and non specific targeting in parasite intracellular. This study was aimed to...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/44467 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Malaria is one of the infectious diseases caused by Plasmodium parasites.
Plasmodium falciparum is the most serious form of the disease causes. The main
problem of malarial treated currently are multiple drug resistance and non specific
targeting in parasite intracellular. This study was aimed to develop a new target
drug therapy for malaria using as-ODN (Antisense Oligodeoxynucleotide)
specifically targeted to eba-175 and dhs genes. As-ODN was encapsulated into
nanoparticles of chitosan and chitosan-poloxamer nanoparticles 10% which were
prepared by using ionic gelation method. The size of chitosan nanoparticles was
70.80-89.17 nm while chitosan-poloxamer nanoparticles were 109.63-178.93 nm
with polidispersity index less than 0.5. Nanoparticles containing as-ODN 0.5 µM
was tested its in vitro activity as antimalaria against Plasmodium falciparum to
determine the inhibition of schizont growth which was observed by microscope.
The inhibition schizont growth produced by chitosan nanoparticles targeted
eba-175 and dhs were 47.7% and 57.2%, while for chitosan-poloxamer
nanoparticles targeted eba-175 and dhs were 58.8% and 68.3%. Respectively, the
inhibition exhibited significantly different between free as-ODN and as-ODN
loaded chitosan and chitosan-poloxamer nanoparticles inhibiting the growth of
schizont Plasmodium falciparum. To identify the linearity between the in vitro
antimalarial activity test result microscopically, total RNA was isolated from
Plasmodium falciparum using TRIzol
TM
LS Reagent. The highest percentation of
the inhibition of schizont growth and the decreation total RNA concentration of
Plasmodium falciparum was given by chitosan-poloxamer nanoparticles with
as-ODN-targeted dhs genes.
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