FORMULATION AND ANTIMALARIAL ACTIVITY TEST OF CHITOSAN BASED NANOPARTICLES CONTAINING ANTISENSE OLIGODEOXYNUCLEOTIDE TARGETED GENES eba-175 AND dhs

• Main Author: Auliasari, Nurul
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44467
• Institution: Institut Teknologi Bandung

Malaria is one of the infectious diseases caused by Plasmodium parasites. Plasmodium falciparum is the most serious form of the disease causes. The main problem of malarial treated currently are multiple drug resistance and non specific targeting in parasite intracellular. This study was aimed to develop a new target drug therapy for malaria using as-ODN (Antisense Oligodeoxynucleotide) specifically targeted to eba-175 and dhs genes. As-ODN was encapsulated into nanoparticles of chitosan and chitosan-poloxamer nanoparticles 10% which were prepared by using ionic gelation method. The size of chitosan nanoparticles was 70.80-89.17 nm while chitosan-poloxamer nanoparticles were 109.63-178.93 nm with polidispersity index less than 0.5. Nanoparticles containing as-ODN 0.5 µM was tested its in vitro activity as antimalaria against Plasmodium falciparum to determine the inhibition of schizont growth which was observed by microscope. The inhibition schizont growth produced by chitosan nanoparticles targeted eba-175 and dhs were 47.7% and 57.2%, while for chitosan-poloxamer nanoparticles targeted eba-175 and dhs were 58.8% and 68.3%. Respectively, the inhibition exhibited significantly different between free as-ODN and as-ODN loaded chitosan and chitosan-poloxamer nanoparticles inhibiting the growth of schizont Plasmodium falciparum. To identify the linearity between the in vitro antimalarial activity test result microscopically, total RNA was isolated from Plasmodium falciparum using TRIzol TM LS Reagent. The highest percentation of the inhibition of schizont growth and the decreation total RNA concentration of Plasmodium falciparum was given by chitosan-poloxamer nanoparticles with as-ODN-targeted dhs genes.