ANALYSIS OF GLYCOPROTEIN IN CANARY (Canarium indicum L.) SEED ENDOSPERM FROM MAKIAN ISLAND, NORTH MALUKU

• Main Author: Nurul Apsari, Cintya
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44515
• Institution: Institut Teknologi Bandung

Canaries (Canarium indicum L.) are widespread commodities in eastern Indonesia, especially Maluku, North Sulawesi and Seram Islands. Research on canary seeds had been done mostly in lipid content, while protein as the second largest component is still unexplored. Therefore, in this study was conducted the analysis of protein in extract and fraction of canary seed endosperm to determine protein profiles therein, and to find out the fraction of proteins that contain glycoprotein, especially lectin. Analysis of canary seed endosperm were from green canary (young) and purple canary (ripe). Protein extraction method was carried out with maseration using NaCl 0,9% and fractionation was done by using ammonium sulfate precipitation with different saturation level, both in endosperm of green canary seed (BKH) and also purple canary seed (BKU). So it was produced five fractions, there were first fraction (saturation 0-20%), second fraction (saturation 20-40%), third fraction (saturation 40-60%), fourth fraction (saturation 60-80%), and fifth fraction (saturation 80-100%). Characterization of extracts and fractions was done with protein content determination, molecular weight determination, glycoprotein identification, and hemagglutination assay. The results of protein content analysis by comparing between BKH and BKU endosperm showed no significant difference between them both on extract and fraction. Electrophoregram profiles showed BKH and BKU endosperm composed of protein with a relatively equal molecular weight, except in 17,4-17,9 kDa, the protein was not found in BKU. Both BKH and BKU endosperm contained four dominant glycoproteins, which had molecular weight of 21,5 –26,8 kDa ; 35,9 –43,4 kDa ; 49,3 –54,8 kDa and 62,6 –67,6 kDa. Hemagglutination assay confirmed that glycoprotein in BKH and BKU endosperm did not hemagglutinate red blood cell, it was suspected that BKH and BKU lectin type was merolectin.