PEMURNIAN DAN KARAKTERISASI SIKLODEKSTRIN-?HASIL PRODUKSI ENZIM SIKLODEKSTRIN GLIKOSILTRANSFERASE REKOMBINAN DARI Bacillus sp. A2-5a DENGAN SUBSTRAT PATI SAGU PADA SKALA LABORATORIUM

• Main Author: Anwar, Alinas
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44544
• Institution: Institut Teknologi Bandung

Cyclodextrin (CD) is a cyclic oligosaccharide molecule consists of glucose monomers which are linked by 1,4-?-ã??®??ð¸ð® ­??¸?=??𸱠ð???®¬?ð??ð?í?¸???í?­ð® ¬?¸ ???í± ????𸱠ð?í?¸???íð?ð®? CD can be used to improve the solubility of hidrofobic compound. CD is produced by enzymatic reaction of the conversion of starch by cyclodextrin glycosyltransferase (CGTase, EC. 2.4.1.19). í±?± ¬?± ?í?±± ???±??» ?????????íð®í ¬?± ?-???????????¬?¸ ??í± ?í¬??¬®±??ð®¬?ð?¸????????¬?????± ?- ????????¸?± ??ð??????®?????» ???¸?®?ð??¬?¸ ?í± ??ð?¬­ð?ð???» ?-CD cavity with many hydrophobic ®??????¸??í± ¬ð??» ?íð????¸?ð???®???¬?± ?í± ?¬?ð???®í¬?¬®?±???» ?-CD which are produce in 1 litre laboratory scale by the enzymatic reaction using recombinant CGTase (rCGTase) of Bacillus sp. A2-5a. An amount of 20 UA crude extract and purified rCGTase enzyme was added into 1% sago ??¬?®í ??­???¬?± ¬??: ????????????»????í????ð?¬ ??¬?ð® ??¬?±???ð»ð®¬?ð???» ?-CD was including the isolation by crystallization and purification by adding cyclohexane as complexing agent. >?ã¬???±??ð® ±?¬??¬?ð???¸±ã?±± ?» ???ð????ð±?¸?¬?¸ ?í± ?¬?ð??» ??????????????±?± determined by UVvis spectrophotometry, high performance liquid chromatography (HPLC), X-ray diffraction (XRD), electrothermal, and a polarized microscopy. ?-CD produced by rCGTase crude extract showed yield ?» ????????????ð?í ???ð???» ???????????±¬??íð?± ?-CD produced by pure rCGTase gave yield of 9.08 ± 2.19 % and purity of 95.57 ± 1.35. The results of HPLC analysis showed retension time of 10-11 ?ð???±?­??-CD from crude and purified rCGTase which are the same as showed by standard ?-CD. The results of PXRD analysis gave specific peaks ¬???= 12.62???????and ?????? ?íð®í ð??¬?± ¬?¸ð»»?¬®??ã?¬??-CD standard. Melting point analysis using electrothermal for ?-CD produced by crude extract, by purified rCGTase and for standard ?-CD were 289.1-289.6 °C, 288.9- 289.6 °C and 289.4-289.9 °C, respectively. Polarized microsope images showed that the crystal of ?-CD from this experiment were flat thin shaped which are similar to standard ?-CD.