FORMULASI DAN EVALUASI NANOEMULSI EKSTRAK PEPAYA (CARICA PAPAYA L.) SERTA UJI AKTIVITAS ANTIOKSIDAN DENGAN 2.2-DIFENIL-1-PIKRILHIDRAZIL

• Main Author: Melati Putri Dewanto, Mekar
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44575
• Institution: Institut Teknologi Bandung

Free radical is molecule or molecule fragment which has unpaired electrons at its outermost orbital. Antioxidant is functioned to exterminate free radicals by reacting and forming stabile molecules. In skin, antioxidant compound has function to eliminate excess ROS (Reactive oxygen species) that is produced and/or potentiate the capacity of endogenous antioxidant. Therefore, antioxidant is a potential therapy for UV damaged skin and UV induced melanoma. Papaya fruit has been reported as a plant that has antioxidant activity. This research was conducted to determine the optimum formula for papaya extract nanoemulsion which also exhibit the optimum antioxidant activity. Materials used in nanoemulsion formulation were VCO (virgin coconut oil), tween 80, span 80, PEG 400, and aquadest. Formula optimization steps included phase selection, surfactant HLB selection, co-surfactant selection, optimization of surfactant, co-surfactant, and extract amount. The conducted steps of nanoemulsion evaluation were organoleptic, particle size, morphology, viscosity, stability, and antioxidant activity assessment by DPPH (2,2-diphenyl-1-picrylhydrazil) inhibition. The obtained optimum formula (W/O nanoemulsion) consisted of 3% papaya extract, 7% aquadest, 8% PEG 400, 17% tween 80, 15% span 80, and 50% VCO. The optimum formula F18 had the viscosity of 276.7 ± 15.3 cps at 50 rpm, the particle size of 115.5 ± 21.9 nm, great stability at freeze and thaw test or temperature stress stability test at 25 and 40 o C, poor stability at centrifuge test, and the DPPH IC50 value of 15.679 ± 3.192 ppm. The nanoemulsion loaded papaya extract revealed greater antioxidant activity than papaya extract.