FORMULASI NANOEMULSI DARI EKSTRAK ETANOL TEH PUTIH (CAMELLIA SINENSIS L.) DAN EVALUASI AKTIVITAS PENYEMBUHAN LUKANYA PADA KELINCI
Tea (Camellia sinensis L.) contains polyphenol compounds which have antioxidant and antiinflammatory activities that have a role in wound healing process. White tea has high levels of polyphenols due to the absence of oxidation process. In this research, white tea extract was formulated into a na...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/44588 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Tea (Camellia sinensis L.) contains polyphenol compounds which have antioxidant and
antiinflammatory activities that have a role in wound healing process. White tea has high levels of
polyphenols due to the absence of oxidation process. In this research, white tea extract was
formulated into a nanoemulsion system to enhance the wound healing activity on the skin. White tea
was extracted by reflux extraction method using 20% ethanol as solvent. Nanoemulsion was
prepared using Tween 80 and Span 80 (1: 1) as surfactants with PEG 400, glycerin, and propylene
glycol as cosurfactants in various ratios by using stirring and sonication methods. Evaluations of
nanoemulsion were conducted on density, globule size, and physical stability by centrifugation,
freeze-thaw? ®®±?±? ?±¸ ?? ð?ð???±?? ??±??±? ???± ???????? ?¸ ?? ð?ð???±?? ??????±??±? ???±?
Evaluation of nanoemulsion was continued by irritation and wound healing activity test using rabbits.
The yield of the extraction was 31.24%, with 46.53% of water soluble extract, 42.86% of ethanol
soluble extract and density of 1% extract was 0.89 g/mL. Formula that produces good nanoemulsion
was containing 0.8% extracts, 8% distilled water, 40.75% mixture of surfactant Tween 80, Span 80
and PEG 400, and 50.45% virgin coconut oil (VCO). The average globule size was 330.90 ± 144.97 nm
with density of nanoemulsion 0.77 ± 0.04 g/mL. Nanoemulsion was stable at room temperature for
four weeks, freeze-thaw test for six cycles, and at temperature 40°C for three weeks. Precipitation
was occurred after 5 hours on centrifugation test. Acute dermal irritation test showed the value of
Primary Irritation Index = 0 which means nanoemulsion did not irritate the skin. There was significant
differences (p <0.05) in wound lengths between control and treated by nanoemulsion on day 4, 6, 7,
and 8, and between extract and nanoemulsion on day 7. The complete wound healing process in
rabbits which was treated by nanoemulsion occurred on day 7, while required 12 days for control
and 9 days for extract to recover completely. Nanoemulsion of white tea extract could improve
wound healing activity in rabbit.
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