UJI AKTIVITAS ANTIBAKTERI DAN UJI ANTI-TOPOISOMERASE I JAMUR HASIL ISOLASI DARI HUTAN BAKAU PANTAI PEJARAKAN BALI

• Main Author: Ahmad Nugraha, Rizki
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44752
• Institution: Institut Teknologi Bandung

Many researches have shown that isolated metabolites from marine fungi have antibacterial and anticancer activity. This research’s purposes were to determine antibacterial activity of isolated fungi from Pejarakan Mangrove Forest against Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus (MRSA) and also determining their anti-topoisomerase I activity. Pure strains were fermented in yeast extract peptone dextrose (YPD) broth for 21 days using shaker with speed of 150 rpm at room temperature. After fermentation had finished, mycelium and broth were separated by vacuum filtration. Mycelium was extracted by maceration and broth was extracted by using liquid-liquid extraction. Extracts were concentrated using rotary evaporator and then the extracts were dried in room temperature. The extracts were tested for their antibacterial activities against Staphylococcus aureus and MRSA. Their antibacterial activities against Staphylococcus aureus and MRSA were determined by agar diffusion method while their minimum inhibitory concentration (MIC) were determined by broth microdilution accompanied by minimum bactericidal concentration (MBC) determination. Anti-topoisomerase I activity of the extracts were determined by measuring their diameter of inhibition against Saccharomyces cerevisiae strain 1140, accompanied by calculating its IC12. Extract mycelium of GK4 showed the biggest antibacterial activity against Staphylococcus aureus (MIC = 256 µg/mL and MBC = 2048 µg/mL) and MRSA (MIC = 1024 µg/mL and MBC = 2048 µg/mL). The result of anti-topoisomerase I activity determination showed that extract mycelium of GK5 showed the biggest activity on inhibiting Saccharomyces cerevisiae’s growth with IC12 = 325,40 µg/mL. Therefore isolated fungi from Pejarakan Mangrove Forest has potential to be antibacteria and anti-topoisomerase I.