PENGEMBANGAN METODE SPEKTROFOTOMETRI SINAR TAMPAK MENGGUNAKAN PEREAKSI JINGGA XILENOL UNTUK PENETAPAN KADAR ZINK DALAM TABLET

• Main Author: Apriyani Yahya, Desy
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44760
• Institution: Institut Teknologi Bandung

Zinc sulfate is used to reduce the severity of diarrhea and administrated with oral rehydration solution. Determination of zinc sulfate in tablet is needed due to efficacy, safety, and quality of dosage form. The purpose of this study was to develop a visible spectrophotometric method for zinc determination. Measurement of zinc by the visible spectrophotometric is based on the formation of colored complexes with the reagent on certain condition. A various parameter such as reagent concentration, pH, temperature, incubation time, maximum wavelength and matrix interferences were optimized to determine optimum condition for reaction. Method validation was performed with the consideration of validation method parameters such as linearity and range, limit of detection (LOD), limit of quantification (LOQ), accuracy and precision. In this study, zinc solution was reacted with xylenol orange reagent 0.0024% w/v at pH 6, incubation allowed for 15 minutes at room temperature and the product was measured at 570 nm. Zinc that measured by this method gave linearity in the range of 1.2 ppm - 4.8 ppm with of 0.999 coefficient of correlation, 1.3% coefficient of variance, 0.137 ppm LOD and 0.415 ppm LOQ. The accuracy value which showed by recovery was in the range of 99.13% - 101.98%. Intraday and interday precision gave the coefficient of variance values less than 2%. Method is used to determine the levels of zinc in commercial tablet. The result of zinc determination in the commercial tablet fulfilled the compendia requirements. The visible spectrophotometric method with xylenol orange had been optimized, validated and could be used to determine the zinc level in tablet.