ISOLASI JAMUR DAN UJI AKTIVITAS ANTIMIKROBA ISOLAT JAMUR DARI HUTAN BAKAU DAERAH KAWASAN EKOLOGI TRITIH KULON CILACAP
Fungi is one of marine microorganism that has great potential to produce active metabolite as antimicrobe. Due to extreme condition of marine ecosystem, marine fungi has potential to produce active metabolite which has stronger activity than terrestrial fungi. The aim of this study was to obtain...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/44862 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Fungi is one of marine microorganism that has great potential to produce active metabolite as
antimicrobe. Due to extreme condition of marine ecosystem, marine fungi has potential to
produce active metabolite which has stronger activity than terrestrial fungi. The aim of this study
was to obtain pure fungal isolates of mangrove forest ecology park area Tritih Kulon, Cilacap,
Central Java and the isolates are tested for antimicrobial activity against Staphylococcus aureus,
Escherichia coli, and Candida albicans. From the research, there are 5 fungal isolates from leaf
samples and 6 fungal isolates from mud samples. Fungal isolates were coded by FTKC 1 to FTKC
11. Then, pure fungal isolates was fermented using a shaker with a speed of 150 rpm for 21 days
and it was separated between the media and the mycelium. The media were extracted by liquidliquid extraction and the mycelium were macerated using ethyl acetate. The results of the
screening antimicrobial activity of the extract with concentration 250 µg/disk are extract media of
FTKC 3, 4, 5, 7 and extract mycelium of FTKC 3, 4, 9 provide antimicrobial activity against S.
aureus. Exract media of FTKC 1, 2, 3, 5, 6, 7, 8, 9, 10, 11 and extract mycelium of FTKC 1, 5, 6, 7, 8,
10, 11 provide antimicrobial activity against E. coli. Extract media of FTKC 11 provides
antimicrobial activity against C. albicans. The lowest MIC value obtained is 128 µg/mL by FTKC 3
extract media culture against S. aureus, 256 µg/mL by FTKC 3 extract media culture against E. coli,
and 1024 µg/mL by FTKC 11 extract media culture against C. albicans. The lowest MBC value
obtained was 256 µg/mL by the FTKC 3 extract media culture against S. aureus. The results
showed that most of the fungal isolates produce the active metabolite which have potential as
antimicrobial agent against Staphylococcus aureus, Escherichia coli and Candida albicans.
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