PENGUJIAN AKTIVITAS DESATURASE DALAM MEDIUM BIAKAN RHIZOPUS ORYZAE TERHADAP SUBSTRAT MINYAK KENARI

• Main Author: Sanjaya Pinem, Destriani
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44937
• Institution: Institut Teknologi Bandung

Canary (Canarium indicum) nut is a source for vegetable oil and has potency to be used widely. Some researchs suggest that oil with high unsaturated fatty acid gives greater benefit for health. Therefore, canary nut oil with higher proportion of unsaturated fatty acid can be used wider in food, medicine, and cosmetics production. Increasing unsaturated fatty acid content can be done using enzyme produced by Rhizopus oryzae. Rhizopus oryzae was grown in oil containing medium in order to produce extracellular enzyme. Canary nut oil and enzyme solution were incubated and optimum time for enzymatic reaction was determined. Fatty acid composition analysis was done using High Performance Liquid Chromatography after hydrolysis and derivatization to p-bromophenacyl ester form. Rhizopus oryzae growth medium was able to increase 2.94% of unsaturated fatty acid in canary nut oil with optimum incubation time was 50 minutes. Therefore, enzyme from Rhizopus oryzae growth medium can be used to increase unsaturated fatty acid percentage in canary nut oil.