UJI AKTIVITAS ANTIMIKROBA BERBAGAI ANTIBIOTIK DAN KOMBINASINYA DENGAN KURKUMIN TERHADAP BAKTERI ESCHERICHIA COLI DAN STAPHYLOCOCCUS AUREUS

• Main Author: Cynthia Setiawan, Isabella
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44973
• Institution: Institut Teknologi Bandung

Bacterial infection is the most common infection that occur in the society. The problems with consuming antibiotics are the possibility of bacterial resistance and a lot of side effects that may occur. Drugs derived from plants are often expected to increase the potency of antibiotics. Turmeric is commonly used traditionally and one of its active substance is curcumin. Based on previous study, curcumin has been proven to have antimicrobial effect and in this research ®??®??ð?????? ??ð?ð®??­ð ?±»»±®?ð?ð??±??ðã ?±¸ ?í±?®??­ð?±¸ ?ð?í ?±?±?al antibiotics to increase their potency, such as neomycin sulphate, chloramphenicol palmitate, ciprofloxacin, trimethoprim, sulfamethoxazole, ampicillin trihydrate and tetracycline hydrochloride. To test the combined effects, three methods were used and the correlations were observed. The first method was the determination of Minimum Inhibitory Concentration(MIC) and Minimum Bactericidal Concentration(MBC) from each antimicrobial substance. The combination effects were determined using paper strip, paper disk and time kill curve. The MICs value of neomycin sulphate, chloramphenicol palmitate, tetracycline hydrochloride, trimethoprim, sulfamethoxazole, ampicillin trihydrate, ciprofloxacin and curcumin towards S.aureus are 8; 32; 0,25; 256; 32; <0,015625; 0,125; 64 µg/mL accordingly. Meanwhile, the MICs value toward E.Coli are 16; 64; 0,5; 64; 32; 8; 0,0625; 64 µg/mL accordingly. The MICs value were used to consider the concentration of antimicrobial agent in the combination test. Based on the combination test toward Escherichia coli, neomycin sulphate, chloramphenicol palmitate, ciprofloxacin, sulfamethoxazole, ampicillin trihydrate and tetracycline hydrochloride combined with curcumin yielded additive antimicrobial effect. Meanwhile, trimethoprim combined with curcumin yielded synergistic effect toward Escherichia coli. The combination of those antibiotics with curcumin yielded additive antimicrobial effect when tested toward Staphylococcus aureus.