AKTIVITAS EKSTRAK ETANOL HERBA PLETEKAN (RUELLIA TUBEROSA L.) DAN DAUN CERMAI (PHYLLANTHUS ACIDUS L. SKEELS) PADA MENCIT DIABETES ALOKSAN

• Main Author: Triseptya Hunto, Stephanie
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44980
• Institution: Institut Teknologi Bandung

Diabetes mellitus ranked second as non-contagious diseases in Indonesia, therefore it is necessary to develop drugs that are capable of treating diabetes. Various herbs have been widely used by the people of Indonesia for treating diabetes and among them are minnie root herbs and star gooseberry leaves. This study aims to examine the antidiabetic activity of the minnie root herbs ethanolic extract (EEHP) and star gooseberry leaves (EEDC). The active ingredients from simplicia minnie root and star gooseberry were extracted by reflux method using ethanol. Activity test carried out on male Swiss Webster mice induced diabetes by alloxan monohydrate through intravenous route and the decrease in blood sugar levels were observed by using glucose strip test. Since alloxan may damage the pancreatic beta cells as free radicals, the antioxidant in the extract was also tested by using DPPH method. Result on DPPH test showed IC50 of minnie root and star gooseberry leaves extracts i.e. 2.57±0.72 and 16.96±0.25 µg/mL, respectively. After 14 days, test on diabetic mice showed EEHP and EEDC at dose of 140 mg/kg body weight can reduced blood glucose level i.e. 43.6 ± 1.5 and 40.6±12.6 %, respectively and at the dose 280 mg/kg body weight can reduced blood glucose level i.e. 37.7±8.5 and 32.9±7.1 %, respectively. Based on this results, it can be concluded that minnie root herbs and star gooseberry leaves ethanolic extract can be developed into the herbal drug to treat diabetes mellitus.