AKTIVITAS ANTIOKSIDAN EKSTRAK TEH OOLONG DARI BANTEN, BENGKULU, DAN JAWA BARAT DENGAN METODE 2,2-DIFENIL-1-PIKRILHIDRAZIL (DPPH) DAN 2,2’-AZINO-BIS(3- ETHYLBENZOTHIAZOLINE-6-SULFONIC ACID (ABTS)

• Main Author: Fitri Pratiwi, Ananda
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44990
• Institution: Institut Teknologi Bandung

Free radicals are atoms or groups of atoms that have one or more unpaired electrons. Free radicals can against and bound with electron of the other molecule. Negative effect of free radical can be inhibited by antioxidant. Antioxidant is compound that can inhibit oxidation reaction by scavenging free radical. Many plants have active compounds which have antioxidant properties such as flavonoid and phenolic compounds. Oolong tea had antioxidant activity. The aim of this research were to determine IC50 of DPPH (2,2-diphenyl-1-picrylhydrazyl) and IC50 ?» !????0 ?????- azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) of Banten, Bengkulu, and West Java oolong tea extract; total phenolic, flavonoid and carotenoid content in various extracts of oolong tea; correlation of total phenolic, flavonoid and carotenoid content with their IC50 of DPPH and IC50 of ABTS scavenging activities, and correlation of two antioxidant methods in sample extracts. Extraction was conducted by reflux using different polarity solvents. Chromatogram pattern on each extracts were observed by thin layer chromatography (TLC). Determination of IC50 of DPPH, IC50 of ABTS, total phenolic, flavonoid and carotenoid content were performed by UV-visible spectrophotometry and its correlation with IC50 of DPPH and IC50 of ABTS scavenging activities ?±?± ? ???±¸ ­?)± ???????±?í?¸?í± íðãí±?? ??ð??ð¸ ?? ®?ð?ð??­?????))???ethod (IC50 0.31 ± 0.01 µg/mL) was showed by ethyl acetate oolong tea extract from West Java, while using ABTS method was given by ethanol oolong tea extract from Banten (IC50 0.02 ± 0.008 µg/mL). All of extracts of oolong tea had linear result in DPPH and ABTS assays. Oolong tea extracts from Banten, Bengkulu and West Java can be categorized as very strong antioxidant. Phenolic compounds were the major contributor in oolong tea extracts from Banten, Bengkulu and West Java. DPPH and ABTS methods gave linear results in all of sample extracts.