PENGARUH HIPERKOLESTEROLEMIA TERHADAP EKSPRESI MYELOPEROKSIDASE PADA AORTA DAN JANTUNG TIKUS WISTAR JANTAN YANG MENGALAMI INFARK MIOKARD

• Main Author: Angelia, Teresa
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/45189
• Institution: Institut Teknologi Bandung

Athesclerotic plaque rupture is the cause of arterial occlusion and interruption of blood and oxygen supply to the myocardium, which results in myocardial infarction. Myocardial infarction is a clinical manifestation of coronary heart disease. Furthermore, biomarkers such as myeloperoxidase (MPO) can give a prognosis of myocardial infarction. MPO is produced by neutrophils and macrophages during an inflammation and is found in various stages of development of atheroma plaques. This study aims to determine the effect of hypercholesterolemia in the inflammatory processes in the aorta and the heart of myocardial infarction rat. First, the animals were classified into a group of normal, normal-myocardial infarction, hypercholesterolemia, and hypercholesterolemia-myocardial infarction. Hypercholesterolemia was induced by feeding high-cholesterol diet as well as the oral administration of cholesterol, cholic acid and propylthiouracil. Model of myocardial infarction was ii made by injecting isoproterenol intraperitoneally, one day before the animals were sacrificed. All lipid profiles were measured in the third month, and total cholesterol were also measured before induction, first and second month after the induction. Successful induction hypercholesterolemia, showed by the increase of total cholesterol significantly in hypercholesterolemia group towards normal group. MPO characterization was then performed by dot blot method. The results showed that in the aorta of hypercholesterolemia rat, MPO expression increased significantly compared to the normal group. The highest expression of MPO in the aorta was observed in hypercholesterolemiamyocardial infarction rat. In the heart organ, myocardial infarction animals and hypercholesterolemia animals showed similar increase of MPO expression (1.7 times higher). However, hypercholesterolemia-myocardial infarction rats still had the highest MPO expression (2.2 times higher). This study suggests that high levels of cholesterol exacerbate inflammation in aorta and heart of myocardial infarction rat which are characterized by the increase of MPO expression.