SNP rs11196205 TRANSCRIPTION FACTOR 7 LIKE 2 (TCF7L2) SEBAGAI MARKER DIABETES MELITUS TIPE 2 PADA ETNIS MINANGKABAU

• Main Author: Badriyya, Elsa
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/45212
• Institution: Institut Teknologi Bandung

Type 2 Diabetes Mellitus (T2DM) is a metabolic disease characterized by elevated glucose levels in the blood, macrovascular and microvascular complication that can cause death in patients. The prevalence of T2DM has been increasing rapidly in developed and developing countries including Indonesian. Province in Indonesian which has high prevalence of T2DM is West Sumatera. Single Nucleotide Polymorphism (SNP) rs11196205 Transcription Factor 7 Like 2 (TCF7L2) gene has been associated with T2DM in several countries like Icelandic, Danish, United States, and Thailand. The aim of this research was to find an association between SNP rs1196205 of TCF7L2 gene with T2DM in ethnic Minangkabau. Design of study was a case control study included 25 T2DM patients and 25 healthy controls who match with the inclusion and exclusion criteria. Amplification Refractory Mutation System Polimerase Chain Reaction (ARMS PCR) was used for identifying TCF7L2 rs11196205 gene polymorphism. Genotype of sample was determined by two ARMS PCR reaction to detect G allele and C allele, produce 3 fragments of 856bp, 559bp, and 339pb. Data was analyzed with chi-square test. Based on the research, C allele and GC genotype were significantly associated to T2DM (p= 0,017 for both analysis). Therefore, SNP rs11196205 TCF7L2 gene can be used as genetic marker of T2DM in ethnic Minangkabau.