DEVELOPMENT OF LOCAL ISOLATE MUSHROOM PRODUCTION TECHNOLOGY Pleurotus giganteus

• Main Author: Utami, Sri
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/45382
• Institution: Institut Teknologi Bandung

Mushroom is a food source contains complete nutrient and beneficials for human health. In the area around Mount Geulis, Sumedang Regency a wild mushroom isolate was found and can be consumed. The mushroom has potential to be developed as a food ingredient. This research aims to develop these mushroom through the optimization of growth media and bacterial induction. The results of the molecular identification by ITS marker indicate that the mushroom isolate is Pleurotus giganteus. The first part of this research was to optimize growth media which include four stages such as stages there are optimization of main substrate (sawdust, cotton waste, and straw), optimization of lime concentration (0.5%; 1%; 1.5%; 2%; 2.5%) (w/w), optimization of the addition of complex carbon sources including type (rice flour, cornstarch, wheat, and tapioca) and its concentration (1%; 3%; 5%) (w/w), and the last was optimization of the addition of complex nitrogen sources including the type (bran and soybean flour) and its concentration (2.5%; 5%; 7.5%; 10%) (w/w). Result showed the most optimal main substrate is a straw, lime concentration of 2% (w/w), addition of cornstarch with a concentration of 5% (w/w), and the addition of rice hull with a concentration of 5% (w/w). The results of the growth media optimization showed a significant increased and it was 0.648 mm/hr, while the growth rate of control was 0.174 mm/hr. Second part was bacterial induction with stages including bacterial isolation, induction test, optimization of bacterial concentration (105; 106; 107; 108; 109 CFU/ml), identification, and optimization of inoculation time (initial inoculation when seedlings, the end of mycelium incubation, and the combined both). Results showed that bacterial isolates induced the growth of mushrooms. The growth rate with the induction of bacteria is 0.370 mm/hr, while the control is 0.168 mm/h. The optimal bacterial concentration for the induction of growth rate is at 108CFU/ml. The molecular identification with the 16S rRNA marker indicates that the bacteria was Bacillus thuringiensis. Results of the optimization of bacterial induction in the media showed that the combined administration resulted in a faster and more fruiting body. For induction treatment, the first harvest time is on the seventh day with BE value of 26.39%. While in the control, the first harvest time is on the fourteenth day with BE value of 8.70%. Also, bacterial induction bacteria tested against the growth of mycelium in the liquid media which stages including optimization of different media (PDB, PGYB, and CB) and co-cultures of fungi and bacteria. Results showed that PDB gives highest dry weight and the co-cultures test showed an increase in mycelium biomass. The results of this research has potential used in the development of Pleurotus giganteus production technology