FORMULASI SEDIAAN ES KRIM MENGANDUNG PROBIOTIK Lactobacillus acidophilus TERENKAPSULASI DAN SERAT GLUKOMANAN

• Main Author: Angelina, Joanne
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/45390
• Institution: Institut Teknologi Bandung

Consumption of probiotic L. acidophilus has many health benefits if it is available in a sufficient amount at the target site. Storage conditions and GI environment might disrupt its viability. Mikroencapsulation is a method that could be applied to maintain the viability of probiotics. Soy milk ice cream is a good carrier for probiotics. Glucomannan fiber has a good water binding capacity, therefore might be usefull as a stabilizer and to prevent crystal growth. This fiber also gives unique textures to the ice cream. Soymilk ice cream was optimized, then the optimized formula used as encapsulated L. acidophilus’carrier. L. acidophilus was encapsulated using sodium alginate, guar gum, and CaCl2 through emulsification method. Microsphere obtained was then evaluated for its mucoadhesive property using falling liquid film method. Its viability through storage condition and in simulated gastrointestinal juice was evaluated using Total Plate Count (TPC) method. Optimum microsphere was obtained from formula of 3% sodium alginate, 1% guar gum, and 0.25M CaCl2. Nude L. acidophillus after 6 hours incubation in Simulated Gastric Juice (SGJ) and Simulated Gut Juice (SGJ) left of 9.47x10 3 CFU/g viable cells, from the origin of 3.75x10 8 CFU/g viable cells. The encapsulated L. acidophilus left of 1.51x10 7 CFU/g viable bacteria from the origin of 3.89x10 8 CFU/g. Mucoadhesive study showed that there was no significant difference (p>0.05) in adhesive strength between those microspheres from all formula. Ice cream formula contained 58.90% soy milk, 25% soybean oil, 0.20 gelatin, 0.25% glucomannan, 0.75% lechitine, 12% sucrose, 2% chocolate powder, and 1% vanilla paste. After 90 days, probiotic product contained nude L. acidophillus left of 6.04x10 5 CFU/g viable cells, from the origin of 7.10x10 8 CFU/g cells. Optimum microsphere formula left of 7.77x10 7 CFU/g viable bacteria from the origin of 9.40x10 8 CFU/g. L. acidophilus microsphere could maintain probiotic’s viability in GI simulated fluid. Innoculated of L. acidophilus microsphere into ice cream could improve the viability during storage. This optimum microsphere has intestinal adhesivity of 71.66 ± 6.21%.