KAJIAN AKTIVITAS INHIBISI HMG-CoA REDUKTASE HASIL HIDROLISIS KOLAGEN KULIT IKAN GABUS (Channa striata) MENGGUNAKAN KOLAGENASE 50 kDa DARI Bacillus licheniformis F11.4

• Main Author: Virginia, Agnes
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/45395
• Institution: Institut Teknologi Bandung

Bioactive peptides produced from enzymatic hydrolysis fibrous proteins have been proved to have several biological activities. Previous study showed that the hydrolysis product of snakehead fish (Channa striata) skin collagen with 26 kDa collagenase from Bacillus licheniformis F11.4 displayed inhibitor HMG-CoA reductase (HMGR) activity. HMGR is one of the enzymes that plays role in cholesterol biosynthesis. The aim of this research was to determine the ability of hydrolysis products of collagen of snakehead fish skin using 50 kDa collagenase from B. licheniformis in inhibiting HMGR activity. Collagen of snakehead fish skin was extracted using acid method and collagenase was produced from B. licheniformis using half-strength Luria Bertani medium containing 5% collagen. Crude collagenase was concentrated and fractionated using DEAE Sephadex A-25 column eluted with increasing gradient concentrations of NaCl. Collagen, collagenase, and fractions were analyzed using SDS-PAGE and collagenolytic activity was analyzed by zymography method. Collagenase of 50 kDa present in fraction 1 was used to hydrolyze the collagen. The reaction was done in 18 hours at 50°C. The hydrolysis product using 3.51 ?g collagen and 9 ng collagenase showed 25.8% inhibition activity against pravastatin. This work for the first time shows that the hydrolysis product of snakehead fish skin collagen and 50 kDa collagenase from B. licheniformis has potential as anticholesterol agent.