FORMULASI SEDIAAN KRIM TRANSFERSOM KAFEIN DAN UJI DIFUSI SEDIAAN SECARA IN VITRO

• Main Author: Zakkaha, Ziyani
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/45404
• Institution: Institut Teknologi Bandung

Cellulite is topographic skin change caused by localized metabolic disorder on subcutaneous tissue with high prevalency in women. Caffeine used to treat cellulite has lipolytic activity on fatty cells. This study aims to develop the formulation to enhance caffeine skin penetration through elastic and ultradeformable (transfersome) vesicle delivery system. Transfersomes consisted of Lecithin S-100 and sorbitan monooleate were prepared by thin film hydration method. The first step was dissolving each component in chloroform and evaporated using rotary evaporator until dry. The thin film was kept in a vacuum overnight and hydrated with phosphate buffer pH 7,4 by rotation using rotary evaporator to form vesicle suspension. After that, the vesicle suspension was sonicated using probe sonicator and extruded through filter membrane 0,2 µm. Furthermore, formulation of transfersome was done by phospholipid and surfactant in ratio 49:1, 87:13, and 4:1 (%w/w). The transfersome of this characterized by determination vesicle size, polydispersity index, entrapment efficiency, deformability, and in vitro diffusion test using Franz cell. The optimum formula for caffeine-loaded transfersome consisted of Lecithin S-100 as phospholipid and sorbitan monooleate in ratio 49:1 (%w/w) and caffeine 0,2%. After optimizing this formula, the result of average particle size of transfersome caffeine was 188,9±4,4 nm with polydispersity index 0,339±0,069 and entrapment efficiency 97,65±0,64%. The difference diffusion test between caffeine-loaded transfersome, caffeine cream, and caffeine-loaded transfersome in cream that could diffuse through membrane were 79,22±0,89%, 20,61±2,76%, and 4,69±0,71%. Caffeine-loaded transfersome in cream significantly enhanced the penetration of membrane shed snake skin compared to the caffeine cream without transfersome (P<0,05).