PENINGKATAN DISPERSIBILITAS NATRIUM ASKORBIL FOSFAT DALAM ASAM OLEAT UNTUK PENGEMBANGAN FORMULA NANOEMULSI TOPIKAL
Sodium ascorbyl phosphate (SAP) has hydrophilic properties (log P = ?0.05) thus it has limited penetration through the skin by topical delivery as an antiaging. Dispersing SAP in oil is an alternative to enhance its penetration through the skin. The objective of this study is to improve dispersib...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/45405 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Sodium ascorbyl phosphate (SAP) has hydrophilic properties (log P = ?0.05) thus it has
limited penetration through the skin by topical delivery as an antiaging. Dispersing SAP in
oil is an alternative to enhance its penetration through the skin. The objective of this study
is to improve dispersibility of SAP in oleid acid that can be developed further into topical
nanoemulsion. Several combinations of surfactant, Tween 80-Span 80, Tween 80-Span 20,
Span 80, and Span 20, were evaluated to increase dispersibility of SAP in oleic acid, that
made by co-lyophilization. The formula of SAP solid-in-oleic acid dispersion that resulted
in transparent dispersion were obtained with addition of Span 80 with ratio 20:1 to the
SAP. Then SAP solid-in-oleic acid dispersion was incorporated into oil phase of
nanoemulsion formula that stabilized by Tween 80 as surfactant and polyethylene glycol
400 (PEG 400) as cosurfactant. SAP solid-in-oleic acid dispersion was evaluated by
measuring antioxidant activity and determining the dispersion type. Nanoemulsion was
evaluated by measuring of globule size and distribution, determining amount of SAP in oil
phase, measuring antioxidant activity using DPPH method, and in-vitro skin penetration
testing by Franz diffusion cell using Python reticulatus membrane. The optimum formula
of nanoemulsion containing SAP solid-in-oleic acid dispersion consisted of oleic acid,
Tween 80, PEG 400, and SAP dispersion in the ratio of 9.4%, 31%, 15%, and 4.8%. The
incorporation of NAP solid-in-oleic acid dispersion into nanoemulsion provided a non
significant globule diameter enhancement from 175.73 ± 23.42 nm to 181.17 ± 9.13 nm
(p>0.05). The scavenging activity of nanoemulsion containing SAP dispersion was 88.07 ±
1.22%. Nanoemulsion containing SAP solid-in-oleic acid dispersion enhanced its
penetration through the skin compared to nanoemulsion containing SAP without dispersing
treatment (p<0.05).
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