UJI AKTIVITAS ANTIMIKROBA DARI PIGMEN BERAS ANGKAK DENGAN METODE BIOAUTOGRAFI
Red yeast rice is rice fermented by Monascus purpureus. Metabolites of Monascus purpureus are potential for anticholesterol and antibacterial agent. The objectives of this research was to analyze Monascus pigment that has antimicrobial activity, using bioautography method. Red yeast rice was extr...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/45420 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Red yeast rice is rice fermented by Monascus purpureus. Metabolites of Monascus
purpureus are potential for anticholesterol and antibacterial agent. The objectives of this
research was to analyze Monascus pigment that has antimicrobial activity, using
bioautography method. Red yeast rice was extracted by ethanol 95% using reflux. Red
yeast rice ethanol extract was fractionated by vacuum column chromatography and
showed three main colours, red, orange, and yellow. The antimicrobial activity were tested
by bioautography method and used Staphylococcus aureus, Escherichia coli, and Candida
albicans as the tested microorganisms. Fractions which showed positive result for
bioautography then refractionated by preparative thin layer chromatography. The
absorbances of the subfractions were measured by UV-Vis spectrophotometry. Yellow
and orange pigments gave inhibition zone against Staphylococcus aureus, but no
inhibition zone against Escherichia coli and Candida albicans. Yellow and orange
pigments of Monascus purpureus on red yeast rice has antibacterial activity.
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