UJI EFEKTIVITAS ANTIMIKROBA FENOKSIETANOL SEBAGAI KANDIDAT PENGAWET VAKSIN HEKSAVALEN SECARA IN VITRO

• Main Author: Annisa Masruroh, Mutiara
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/45436
• Institution: Institut Teknologi Bandung

Phenoxyethanol which has antimicrobial activity was chosen as preservative in development of a multi-dose hexavalent vaccine formulation produced by PT. Biofarma. The objective of this study was to obtain effective concentration of phenoxyethanol as hexavalent vaccine preservative based on United States Pharmacopeia (USP) requirements. Susceptibility of three viable microorganisms: Escherichia coli ATCC 8739, Staphylococcus aureus ATCC 6538, and Candida albicans ATCC 10231 was tested toward hexavalent vaccine containing phenoxyethanol at three concentrations of 0,5; 0,75; and 1% v /v. Initial inoculum was prepared by adjusting suspension to achieve a turbidity equivalent to 0,5-1 McFarland standard for bacteria and 5 McFarland standard for yeast and tested using total plate count method. Culture challenges were exposed to the vaccine sampel containing various concentration of phenoxyethanol, and the antimicrobial effect of the solution was determined using plate count method. Log10 reduction rate of each viable count then calculated at 0, 1, 7, 14, and 28 days. Phenoxyethanol met the requirements of AME test upon E. coli and C. abicans at a concentration of 0,5; 0,75; and 1%, while it only met the acceptance criteria at concentration of 1% on S. aureus. The effective concentration of phenoxyethanol as hexavalent vaccine preservative based on antimicrobial effectiveness test toward three culture challenges was 1% v /v.