PENGEMBANGAN METODE ANALISIS KADAR ETAMBUTOL, ISONIAZID DAN PIRIDOKSIN DALAM SEDIAAN TABLET KOMBINASI DENGAN METODE SPEKTROFOTOMETRI UV-VIS
Tuberculosis is a high prevalence disease. Treatment for tuberculosis is using several combination of active substance. Its therapy also must be carried out continously in long term treatment. In order to improve compliance and ease of use, developed dosage form containing combination of several...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/45448 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Tuberculosis is a high prevalence disease. Treatment for tuberculosis is using
several combination of active substance. Its therapy also must be carried out
continously in long term treatment. In order to improve compliance and ease of
use, developed dosage form containing combination of several active substance.
The development of those combined dosage must be followed by the development
of its analytical methods. An analytical method on research laboratory has to be
modified or developed in order to obtain a better analytical method.
Spectrophotometry commonly used in determining the level of active substance in
a dosage. The development of analytical method must satisfy to the parameter of
validation and criteria. These parameter are linearity, limit of detection, limit of
quantization, accuracy, and precision. The result of this research and development
shows that the correlation coefficient of ethambutol, isoniazid, and pyridoxine
consecutively are 0.9994, 0.99975 and 0.99972, respectively. The limit of
detection of ethambutol, isoniazid, and pyridoxine are 0.318, 1.641, and 0.123
µg/mL respectively. The limit of quantization of ethambutol, isoniazid, and
pyridoxine consecutively are 1.062, 5.470, and 0.411 µg/mL respectively. The
recovery value on accuracy testing of ethambutol is 97.5-103.55%. The recovery
value of isoniazid is 100-103.44%. The recovery value of pyridoxine is 97.6-
102.7%. The coefficient of variance on precision testing of ethambutol, isoniazid
and pyridoxine are 0.56%, 1.53% and 1.26% respectively. Concentration of
ethambutol, isoniazid dan pyridoxine in samples are 99.97%, 101.07%, dan
96.67% respectively. Based on test results, it can be concluded that the developed
method can be applied for analysis of ethambutol, isoniazid, and pyridoxine in
combined dosage tablet.
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