PENGEMBANGAN METODE ANALISIS KADAR ETAMBUTOL, ISONIAZID DAN PIRIDOKSIN DALAM SEDIAAN TABLET KOMBINASI DENGAN METODE SPEKTROFOTOMETRI UV-VIS

• Main Author: N Atikasari, Nadilla
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/45448
• Institution: Institut Teknologi Bandung

Tuberculosis is a high prevalence disease. Treatment for tuberculosis is using several combination of active substance. Its therapy also must be carried out continously in long term treatment. In order to improve compliance and ease of use, developed dosage form containing combination of several active substance. The development of those combined dosage must be followed by the development of its analytical methods. An analytical method on research laboratory has to be modified or developed in order to obtain a better analytical method. Spectrophotometry commonly used in determining the level of active substance in a dosage. The development of analytical method must satisfy to the parameter of validation and criteria. These parameter are linearity, limit of detection, limit of quantization, accuracy, and precision. The result of this research and development shows that the correlation coefficient of ethambutol, isoniazid, and pyridoxine consecutively are 0.9994, 0.99975 and 0.99972, respectively. The limit of detection of ethambutol, isoniazid, and pyridoxine are 0.318, 1.641, and 0.123 µg/mL respectively. The limit of quantization of ethambutol, isoniazid, and pyridoxine consecutively are 1.062, 5.470, and 0.411 µg/mL respectively. The recovery value on accuracy testing of ethambutol is 97.5-103.55%. The recovery value of isoniazid is 100-103.44%. The recovery value of pyridoxine is 97.6- 102.7%. The coefficient of variance on precision testing of ethambutol, isoniazid and pyridoxine are 0.56%, 1.53% and 1.26% respectively. Concentration of ethambutol, isoniazid dan pyridoxine in samples are 99.97%, 101.07%, dan 96.67% respectively. Based on test results, it can be concluded that the developed method can be applied for analysis of ethambutol, isoniazid, and pyridoxine in combined dosage tablet.