HEPATOPROTEKTIF KOPI ARABIKA DAN KOPI ROBUSTA PADA TIKUS WISTAR BETINA YANG DIINDUKSI DENGAN PARASETAMOL

• Main Author: Vanesia S, Putri
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/45473
• Institution: Institut Teknologi Bandung

For the last twenty years some researchers reported that drinking coffee may protect the liver. Research was carried out in the form of a questionnaire conducted on patients with liver diseases such as hepatitis, cirrhosis, and liver cancer. The results showed an improvement to the condition of the liver in patients who regularly drink coffee. Nevertheless, the type of coffee used in providing protection against the liver are still unclear. This research was carried out to examine the hepatoprotective activity of Arabica coffee and Robusta coffee in female Wistar rats induced with Paracetamol. Paracetamol is a para-aminophenol derivative drugthat can cause liver damage when is used in high dose or in the long term. Measurement of the enzyme activity of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were conducted to detect any damage to the liver.Arabica and Robusta-brewed coffee and silymarin extract are given to female Wistar rats for 21 days consecutively. On day 7 to day 14, rats are induced by high dose of paracetamol (1200mg/kg) 3 hours after the administration of the coffee and silymarin extract. Parameters determined included the activity of the enzyme AST and ALT, liver index, as well as histopathological examination of liver cells.Significant liver damage, after the induction of Paracetamol for 14 days, is observedin the control positive group through monitoring of AST and ALT activity. AST and ALT activity increased to 29,5% and 79,34% respectively (based on statistical tests using ANOVA, p<0,05). Arabica coffee at the dose of 765mg/kg were able toprevent liver damage significantly compared to the control positive group by using ANOVA test (p<0,05). However, Robusta coffee cannot prevent liver damage at the same dose as Arabica coffee. On the 21 st day, the liver index of the group given with Arabica coffee and Robusta coffee showed no significant difference to the control positive group and the normal group. Based on the activity monitoring of AST and ALT, brewed Arabica coffee at the dose of 765mg/kg can prevent liver damage due to the use of high doses of paracetamol (1200mg/kg).