Isolasi Golongan Flavonoid dari Daun Kacang Babi (Tephrosia vogelii J. D. Hooker)

• Main Author: Nur Alam, Mukharij
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/45499
• Institution: Institut Teknologi Bandung

Tephrosia leaves (Tephrosia vogelii J. D. Hooker) commonly used in treatment for many diseases, agriculture, and livestock. According to WHO, people used to take this plant to cure many diseases. Meanwhile specifically in Indonesia use these species for bio pesticide and livestock as. Rarely happened experiment use these species in Indonesia. Flavonoid that included in Tephrosia are quersetin, flavanones, isoflavones, flavonols, and rotenoids. The aim of this research was to isolate flavonoid compound from Tephrosia vogelii leaves. Crude drug was extracted by reflux using methanol as solvent. Methanol extract was fractionated by liquid –liquid extraction method (charaux-paris method) and using radial chromatography. Purification was done by dissolving targeted compound using suitable solvent. Isolate was characterized by spectrophotometer UV –visible with shifting reagent and spectrophotometer IR. Phytochemical screening indicated that crude drug of Tephrosia vogelii leaves contained alkaloid, flavonoid, and steroid/triterpenoid. Methanol extract contained alkaloid and flavonoid. Isolated compound gave positive result with citroboric and AlCl3 reagent. The UV spectrum of methanolic solution of isolate exhibited two major absorption bands at 312 nm and 270 nm. After addition of shifting reagent the wave length shifted to 317 nm and 275 nm when it was added with sodium acetate. In other hand, the result of spectrophotometer IR had hydroxyl group at 3000 –3300 cm -1 , C=O at 1700 cm -1 , and aromatic group at 1500 cm -1 . Isolated A was proposed as isoflavone with hydroxyl group at C-7.