THE EFFECT OF VEGETABLE OILS OXIDATION AS LICORICE FLAVONOID OIL EXCIPIENT TO GLABRIDIN MARKER CONTENT

• Main Author: Nurul Wasiah Bachtiar, Lisma
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/45719
• Institution: Institut Teknologi Bandung

Obesity is a medical condition with excessive body fat. Excess in visceral fat is closely related to type 2 diabetes mellitus. The use of licorice flavonoid oil (LFO) shows a significant decrease in visceral fat. LFO uses a saturated oil named medium chain triglyceride (MCT) as filler oil. In this research, MCT as filler oil was replaced by several saturated fatty acid contained vegetable oils then their oxidative stability was observed by determining glabridin marker. Analytical method of determination glabridin content was validated by using high performance liquid chromatography at 230 nm. This validation fulfilled parameters of specificity, accuracy, repeatability, linearity and robustness (correlation coefficient is 0.988 at range of 80 ppm and 120 ppm). Limit of detection and limit of quantification are 2.53 ppm and 7.66 ppm respectively. In stability study, peroxide value of MCT (control) was not increased significantly at three weeks storing in closed container, open container and open container added with 0.1 mg copper per kg oil. Peroxide value of olive oil was not increased significantly at one week storing in closed container, but it was increased statistically significant (p < 0.05) at two weeks storing in open container and open container added with copper. Peroxide value of corn oil, soybean oil, canola oil, rice bran oil, sunflower oil and grape seed oil was increased statistically significant (p < 0.05) at one week storing in closed container, open container and open container added with copper. Concentration of glabridin in MCT (control), olive oil, corn oil, soybean oil, canola oil, rice bran oil and sunflower oil was not significantly decrease at three weeks storing in closed container, open container and open container added with 0.1 mg copper per kg oil. In grape seed oil, concentration of glabridin was not significantly decrease at three weeks storing in closed container, but it was statistically significant (p < 0.05) decrease at three weeks storing in open container and open container added with 0.1 mg copper per kg oil. This research showed that olive oil, corn oil, soybean oil, canola oil, rice bran oil and sunflower oil have potential as LFO filler oil.