MICROEMULSION TOPICAL FORMULATION OF ALLANTOIN AND DIFFUSION TEST FOR IN VITRO PERCUTANEOUS
Allantoin is an active substance that is present in a topical preparation. Recent research states that allantoin can increase the synthesis of pro-collagen. Because of the solubility properties owned allantoin, it will be difficult to pass through the stratum corneum layer which has a hydrophilic...
Saved in:
| Main Author: | |
|---|---|
| Format: | Theses |
| Language: | Indonesia |
| Subjects: | |
| Online Access: | https://digilib.itb.ac.id/gdl/view/45734 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Allantoin is an active substance that is present in a topical preparation. Recent
research states that allantoin can increase the synthesis of pro-collagen. Because
of the solubility properties owned allantoin, it will be difficult to pass through the
stratum corneum layer which has a hydrophilic and hydrophobic part that is brick
and mortar. The purpose of this study is to produce a formula allantoin in the
microemulsion with type W/O that contains Virgin Coconut Oil (VCO), Tween 80,
polyethyleneglycol 400 and glycerin. In vitro diffusion test, Batik Python snake
skin used as a membrane. Method for making a microemulsion in this study by
using the titration method and the Phase Inversion Temperature (PIT). Tools used
to analyze the size of the globule is Transmission Electron Microscopy (TEM).
Determination of levels of allantoin in microemulsion W/O using the method of
High Performance Liquid Chromatography. From this study obtained two
formulas microemulsion W/O are E.2.2 and E.2.3. Its have a range globule size
between 130-500 nm. Based on t.tes-paired test using Microsoft Excel, to test the
in vitro diffusion that differ significantly for the second formula in the 15
th
minutes. The E.2.2 formula allantoin in the 15
th
minutes to diffuse as much as
36.60 ± 51.40 mg/cm
2
, while the formula E.2.3 diffused as much as 267.36 ±
27.94 mg/cm
2
. Based on the results of the statistical analysis, it can be concluded
that the formula is a formula E.2.3 better than formula E.2.2. The E.2.3 Formula
consists of 0.5% allantoin, 12% aquadeion, 28.7% glycerol, 36% Tween 80, 1.2%
PEG 400 and 21% VCO. In the stability test for 30 days with the parameters of
pH and viscosity, also the 29 days to content determination parameter, statistical
tests t.test-pairs, there was no significant difference for the both of formulas.
|
|---|