KAJIAN SELEKTIVITAS NANOEMULSI KURKUMIN TERHADAP VIRUS DENGUE SEROTIPE 1 DAN 2
Curcumin is found in the Zingiberaceae family which is a group of active compounds from the curcuminoid group. Curcumin has been widely used as an active ingredient that has the potential as an antioxidant, anticancer, anti-inflammatory, antimicrobial, antifungal and antiviral. The main constrain...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/46660 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Curcumin is found in the Zingiberaceae family which is a group of active compounds from the
curcuminoid group. Curcumin has been widely used as an active ingredient that has the potential as
an antioxidant, anticancer, anti-inflammatory, antimicrobial, antifungal and antiviral. The main
constraints in the development of curcumin preparations are their low solubility in water, degraded by
light and temperature, easily metabolized to inactive forms that causing low bioavailability of the body,
and high metabolism in the liver. Curcumin has been tested to determine the effect of dengue
antivirus, specifically on the DENV-2. The dengue virus has a genome that encodes three structural
proteins and seven nonstructural. This study aims to develop nanoemulsion containing curcumin
(curcumin nanoemulsion) and evaluate physical characteristics, cytotoxicity effects of curcumin on
A549 cells and selectivity of inhibitory effects on dengue virus in serotypes 1 and 2. The cell cytotoxicity
effect of curcumin nanoemulsion was determined using the MTT assay on the A549 human lung cancer
cell line to obtain a CC50 value, compared with a curcumin solution in DMSO. Inhibitory effect on DENV1 and DENV-2 virus titers with the RT-qPCR method to obtain an IC50 value. Curcumin nanoemulsion
was successfully formed by emulsification process, resulting in nanoemulsion with a uniform droplet
size distribution. The results of the droplet size characterization of curcumin nanoemulsion were 24,8
± 2.5 nm, the polydispersity index of curcumin nanoemulsion was 0.189 ± 0.385 and the potential zeta
of curcumin nanoemulsion was -4.3 ± 0.5 mV. The morphology of encapsulated curcumin in the
nanoemulsion system has a spherical and uniform shape. The average percentage of the loading
capacity of curcumin was 93.750 ± 0.950%. The CC50 value of curcumin nanoemulsion obtained was
21,09 ± 1,38 µg/ml, while the CC50 value of curcumin solution obtained was 33,66 ± 5,91 µg/ml. IC50
value of curcumin nanoemulsion was 1.688 µg/ml for DENV-1 and 4.809 µg ml for DENV-2. Curcumin
in the form of nanoemulsion and solution in DMSO is better in reducing DENV-1 virus titer compared
to DENV-2. However, curcumin in the form of nanoemulsion and solution is very responsive in
decreasing the virus titer in DENV-1 and DENV-2.
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