ANTIOXIDANTS ACTIVITIES OF MULTIFLORA HONEY FROM APIS CERANA BEE USING DPPH AND FRAP METHODS

• Main Author: Made Dwi M. Widyani Nayaka, Ni
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/46794
• Institution: Institut Teknologi Bandung

Background and purposes: Honey is widely used as a natural source of antioxidant. Phytochemical characteristic and bioactivities of honey depend on their collected location, flower sources, bee species and industrial processes. The purpose of this study was to determine antioxidant activities of Apis cerana honey from Badung, Karangasem and Singaraja Districts in Bali Province, Indonesia by using DPPH and FRAP methods. Also, total phenolic and flavonoid content of honey samples were determined, and correlations between these parameters were evaluated. Methods: Honey was extracted by reflux technique consecutively using n-hexane, ethyl acetate then ethanol and directly using ethanol. Antioxidant activities, total phenolic and flavonoid were identified with UV-Vis spectrophotometer. In addition, antioxidant activitiy index (AAI) of honey extracts were determined. Correlation of total phenolic and flavonoid content with AAI of DPPH and FRAP also the relationship between two antioxidant methods were analyzed by Pearson’s method. Result: The highest antioxidant activities by DPPH and FRAP were shown by ethyl acetate extract of Karangasem honey with AAI values were 33.521 and 4.874 respectively. This sample also showed the highest content of phenolic (4.327 g GAE/100 g) and flavonoid (2.053 g QE/100 g). Total phenolic content of all samples had positively and significant correlation with their AAI of DPPH and FRAP. AAI of DPPH from Karangasem and Singaraja honey gave positively and significant correlation with their AAI of FRAP. Conclusions: All extracts had antioxidant activities. Phenolic compounds mainly contributed in antioxidant activities of honey extracts by DPPH and FRAP methods. DPPH and FRAP methods showed liniear results in antioxidant activities of honey extracts from Karangasem and Singaraja.