DEVELOPMENT OF A PEPTIDE-BASED DIAGNOSTIC KIT BY USING INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) METHOD IN THE DETECTION OF HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 (HIV-1)
HIV-1 is a virus belonging to the genus Lentivirus, family Retroviridae. This virus can attack CD4+ T cells in humans. Long-term infection of HIV-1 can cause the condition of AIDS. This condition is characterized by the number of CD4+ T cells less than 200 cells / ml of blood, rapidly increasing...
Saved in:
| Main Author: | |
|---|---|
| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/48428 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | HIV-1 is a virus belonging to the genus Lentivirus, family Retroviridae. This virus
can attack CD4+ T cells in humans. Long-term infection of HIV-1 can cause the
condition of AIDS. This condition is characterized by the number of CD4+ T cells
less than 200 cells / ml of blood, rapidly increasing virus particles, and occurring
opportunistic diseases. AIDS has become a pandemic in the world. There is an
urgency to develop diagnostic kits that can detect HIV-1 for monitoring and
controlling the spread of HIV. HIV detection generally uses serologic test, like
ELISA. This study aims to determine the antigenicity of the p17 HIV-1 epitope by
method Indirect Enzyme-Linked Immunosorbent Assay (ELISA) against serum of
HIV positive patients. In this study, negative control is used in the form of blood
serum from healthy individuals and patient samples that is originating from Stored-
Biological Material of 14 HIV patients at Hasan Sadikin General Hospital, West
Java, Indonesia. In this study, the ELISA test was also carried out with a
commercial ELISA kit on negative controls and patient samples to ensure negative
controls and patient samples are used on an assay developed in good condition so
that it functions whether or not the developed assay system can be determined. The
epitope in PBS buffer solution was coated to microplate well at 40C. The incubation
time is 16 hours. In this study, the presence of epitopes from the concentration range
of 2500, 2000, 1500, 1000, 500, 100, 50, 10, 5, and 1 ng epitope / wells can still be
recognized by HIV patient antibodies. This shows the epitope p17 had antigenicity
against anti-HIV antibodies in patient sera samples quantitatively.
|
|---|