DEVELOPMENT OF A PEPTIDE-BASED DIAGNOSTIC KIT BY USING INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) METHOD IN THE DETECTION OF HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 (HIV-1)

• Main Author: Adi Sadewa, Alfonsus
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/48428
• Institution: Institut Teknologi Bandung

HIV-1 is a virus belonging to the genus Lentivirus, family Retroviridae. This virus can attack CD4+ T cells in humans. Long-term infection of HIV-1 can cause the condition of AIDS. This condition is characterized by the number of CD4+ T cells less than 200 cells / ml of blood, rapidly increasing virus particles, and occurring opportunistic diseases. AIDS has become a pandemic in the world. There is an urgency to develop diagnostic kits that can detect HIV-1 for monitoring and controlling the spread of HIV. HIV detection generally uses serologic test, like ELISA. This study aims to determine the antigenicity of the p17 HIV-1 epitope by method Indirect Enzyme-Linked Immunosorbent Assay (ELISA) against serum of HIV positive patients. In this study, negative control is used in the form of blood serum from healthy individuals and patient samples that is originating from Stored- Biological Material of 14 HIV patients at Hasan Sadikin General Hospital, West Java, Indonesia. In this study, the ELISA test was also carried out with a commercial ELISA kit on negative controls and patient samples to ensure negative controls and patient samples are used on an assay developed in good condition so that it functions whether or not the developed assay system can be determined. The epitope in PBS buffer solution was coated to microplate well at 40C. The incubation time is 16 hours. In this study, the presence of epitopes from the concentration range of 2500, 2000, 1500, 1000, 500, 100, 50, 10, 5, and 1 ng epitope / wells can still be recognized by HIV patient antibodies. This shows the epitope p17 had antigenicity against anti-HIV antibodies in patient sera samples quantitatively.