DEVELOPMENT OF ELISA READER INSTRUMENT FOR DIAGNOSING HEPATITIS B VIRUS INFECTION USING LED AS A LIGHT SOURCE
Elisa method (enzyme-linked immunosorbent assay) is a gold standard for diagnosing Hepatitis B Virus infection in Human. The method need three component for diagnosing, the component are blood sample / serum from the patient, diagnostic kit and ELISA reader instrument. This research designed an...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/52260 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Elisa method (enzyme-linked immunosorbent assay) is a gold standard for
diagnosing Hepatitis B Virus infection in Human. The method need three
component for diagnosing, the component are blood sample / serum from the
patient, diagnostic kit and ELISA reader instrument. This research designed an
ELISA reader that is composed of an LED connected to an optical fiber as its light
source, silicone photodiode as its sensor, stepper motor as wellplate driver and
microcontroller as controller. This research uses one LED lamp with a wavelength
of 450 nm. A mechanical construction is designed to connect LED lamps with
optical fiber. The purpose mechanical construction are to split one light source into
eight light sources, so that each sensor receives the same wavelength. A total of
eight SFH213 photodiode sensors were used for measurements. The measurement
validation was carried out by using the benchmarking method against commercial
ELISA readers. The commercial ELISA reader used is iMark from Biorad. The test
results prove that the ELISA reader being designed could measure from 0 OD to
1,6 OD with an error of 0.093 ± 0.098 OD. Based on these results, the ELISA reader
meets the specifications to be used to detect hepatitis B virus infection. |
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