PENETAPAN KADAR KETAMIN HIDROKLORIDA DALAM SEDIAAN INJEKSI DENGAN METODE KROMATOGRAFI CAIR KINERJA TINGGI
<br /> An ion pair high performance liquid chromatographic method was developed for determination of ketamine hydrochloride in injection dosage form. Result showed that high performance liquid chromatographic method using a mixture of acetonitrrle-0.05 M phosphate buffer of pH 4.5 containing...
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| Format: | Theses |
| Language: | Indonesia |
| Subjects: | |
| Online Access: | https://digilib.itb.ac.id/gdl/view/5255 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | <br />
An ion pair high performance liquid chromatographic method was developed for determination of ketamine hydrochloride in injection dosage form. Result showed that high performance liquid chromatographic method using a mixture of acetonitrrle-0.05 M phosphate buffer of pH 4.5 containing 0.05% sodium hexane sulphonate (25:75) as mobile phase, flow rate of 1.0 mL/minute, C-18 column, and detection wavelength of 215 nm. The method validation with an external standard showed that the accuracy was 99.50±0.66 %, the limit of detection and limit of quantitation were 0.93 and 3.09 pg/mL, respectively. The method validation with atropine sulphate as an internal standard showed that the accuracy was 100.84±1.92 %, the limit of detection and limit of quantitation were 1.46 and 4.87 gg/mL, respectively. |
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