ANTI-ANGIOGENESIS ACTIVITY FRACTION AND ISOLATED FROM OF LEMPUYANG WANGI RHIZOME (ZINGIBER ZERUMBET L.) ETHANOLIC EXTRACTS IN VIVO USING ZEBRAFISH (DANIO RERIO) EMBRYOS
Angiogenesis is the growth of new blood vessel from an existing boold vessel. Pathological conditions it can cause rheumatoid arthritis, psoriasis, and cancer. Cancer is a disease caused by uncontrolled cell growth, and has the ability to offend other tissues and metastasize. Zerumbone is one suc...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/53379 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Angiogenesis is the growth of new blood vessel from an existing boold vessel.
Pathological conditions it can cause rheumatoid arthritis, psoriasis, and cancer.
Cancer is a disease caused by uncontrolled cell growth, and has the ability to
offend other tissues and metastasize. Zerumbone is one such compound isolated
from lempuyang wangi rhizome that possesses diverse pharmacological
properties including those of anti-bacterial, anti-oxidant, and anti-inflammatory.
Zerumbone has shown its anti-cancer effects by causing significant suppression
of proliferation, angiogenesis, and apoptosis. The objective of this study was to
the acute toxicity effects and anti-angiogenesis activity of zerumbone fractions
and isolated from of lempuyang wangi rhizome ethanolic extract. Acute toxicity
testing is done static method, referring to OECD Protocol N0 236 year 2013,
while for anti-angiogenesis studies using zebrafish embryos with the endogenous
alkaline phosphatase (EAP) staining method. The results of the acute toxicity
testing obtained LC50 values at 96 hours after fertillization (hpf) water fraction
(LWFA) 445,214 µg/mL, the ethyl acetate fraction (LWFE) 3,470 µg/mL, the n-
Hexane fraction (LWFN) 1,663 µg/mL and the isolate zerumbone (Zer) 1,598
µg/mL. From the results of anti-angiogenesis tests LWFA, LWFE, LWFN, and
Zer had anti-angiogenetis activity by inhibiting the formation of interconnection
vessels (ICV), subintestinal vein (SIV), and ventral posterior cardial distance
(vPCV) to SIV.
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