ANTI-ANGIOGENESIS ACTIVITY FRACTION AND ISOLATED FROM OF LEMPUYANG WANGI RHIZOME (ZINGIBER ZERUMBET L.) ETHANOLIC EXTRACTS IN VIVO USING ZEBRAFISH (DANIO RERIO) EMBRYOS

• Main Author: Septi Lestari, Kharina
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/53379
• Institution: Institut Teknologi Bandung

Angiogenesis is the growth of new blood vessel from an existing boold vessel. Pathological conditions it can cause rheumatoid arthritis, psoriasis, and cancer. Cancer is a disease caused by uncontrolled cell growth, and has the ability to offend other tissues and metastasize. Zerumbone is one such compound isolated from lempuyang wangi rhizome that possesses diverse pharmacological properties including those of anti-bacterial, anti-oxidant, and anti-inflammatory. Zerumbone has shown its anti-cancer effects by causing significant suppression of proliferation, angiogenesis, and apoptosis. The objective of this study was to the acute toxicity effects and anti-angiogenesis activity of zerumbone fractions and isolated from of lempuyang wangi rhizome ethanolic extract. Acute toxicity testing is done static method, referring to OECD Protocol N0 236 year 2013, while for anti-angiogenesis studies using zebrafish embryos with the endogenous alkaline phosphatase (EAP) staining method. The results of the acute toxicity testing obtained LC50 values at 96 hours after fertillization (hpf) water fraction (LWFA) 445,214 µg/mL, the ethyl acetate fraction (LWFE) 3,470 µg/mL, the n- Hexane fraction (LWFN) 1,663 µg/mL and the isolate zerumbone (Zer) 1,598 µg/mL. From the results of anti-angiogenesis tests LWFA, LWFE, LWFN, and Zer had anti-angiogenetis activity by inhibiting the formation of interconnection vessels (ICV), subintestinal vein (SIV), and ventral posterior cardial distance (vPCV) to SIV.