AKTIVITAS ANTIBAKTERI KOMBINASI EKSTRAK ETANOL DAUN SIRIH HIJAU (PIPER BETLE L.) DAN DAUN SENGGUGU (CLERODENDRON SERRATUM (L) MOON.)TERHADAP MIKROBA PENGINFEKSI RONGGA MULUT

• Main Author: Fazriya Hayati, Silmi
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/54864
• Institution: Institut Teknologi Bandung

The oral cavity is a part of body that suitable for growth of microorganism such as bacteria. Bacteria that grow in the oral cavity can cause oral infections with the symptoms bad breath, dental caries, parotitis, etc. In previous studies, betle leaves (Piper betle L.) and senggugu leaves (Clerodendron serratum (L) Moon.) showed antibacterial activity against some oral bacteria. Besides, betel leaves been widely used to maintain the health and hygiene of the oral cavity. The objective of this research was to determine the type combination of betle leaves extract and senggugu leaves extract against bacteria that infected oral cavity including Streptococcus mutans and Staphylococcus aureus using in vitro method. The minimum inhibitory concentration (MIC) of both extracts against Streptococcus mutans and Staphylococcus aureus bacteria were determined by microdilution method and minimum bactericidal concentrations (MBC) were determined by agar diffusion. The results showed that the MIC and MBC value of betel leaves extract against Streptococcus mutans were ????g?mL and ?????g?mL, respectively. The MIC and MBC values were same against Staphylococcus aureus. The MIC and MBC value of senggugu leaves extract were ????g?mL and > ?????g?mL against Streptococcus mutans. The MIC and MBC values of sengugu extract were ?????g?mL and ?????????g?mL againts Staphylococcus aureus. The characteristics of extract combinaton were determined by paper ribbon method, time kill curve and checkerboard microdilution. Based on the result of interaction test between betel leaves and senggugu leaves extract against Streptococcus mutans and Staphylococcus aureus bacteria were additive, that the antimicrobial effect is the sum of each single effect.