AKTIVITAS PENURUN TEKANAN DARAH EKSTRAK ETANOL DAUN PEPAYA (CARICA PAPAYA L.) PADA TIKUS SPRAGUE DAWLEY JANTAN
Hypertensionis a condition when the blood pressure increase above normal, it can develop into risk factors for other disease, such as heart disease and stroke if not handled properly. Treatment of hypertension from natural products can be used as an alternative. Papaya leaves contain carpaine, an...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/54891 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Hypertensionis a condition when the blood pressure increase above normal, it can develop
into risk factors for other disease, such as heart disease and stroke if not handled properly.
Treatment of hypertension from natural products can be used as an alternative. Papaya
leaves contain carpaine, an alkaloidthat has effect on lowering blood pressure. This study
aims to determine the blood pressure-lowering activity of ethanolic extract of papaya
(Carica papaya L.) leaves in Sprague Dawley male rats to test the diuretic and
antihypertensive activity with adrenalineand NaCl inductors. Diuretic activity tested with
metabolic cages performed in the control group, furosemide 1,8 mg/kg bw, and extract
doses of 125 mg/kg bw, 250 mg/kg bw, and 500 mg/kg bw. The entire group was given a
50 mL/kg physiological saline as loading dose. Except for control group, it also given a
dosage of furosemide or test extract. Parameter of percent volumetric urinary excretion
was observed every hour for 6 hours and accumulated up to 24 hours. Antihypertensive
activity tested with adrenaline and NaCl inductors made on the negative control, positive
control, and extract with three different doses and comparative, respectively, atenolol 4,5
mg/kg bw and captopril 13,5 mg/kg bw. Parameters such as amplitude and heart rate
(frequency) at adrenaline induction observed in T-0 before the given dosage, T-1 after a
given dosage, as well as T-2 after adrenalin induction intraperitoneally, while the
parameters of NaCl induction observed at day 0 before induction, day 14 after NaCl
induction orally, and day 28 after induction and therapy. Diuretic test showed that
ethanolic extract of papaya leaves have diuretic effect on doses of 125 mg/kg bw, 250
mg/kg bw, and 500 mg/kg bw in compare to furosemide 1,8 mg/kg bw on the 2nd hour of
observation. Antihypertensive test with adrenaline induction showed the effect of
decreasing the amplitude of the heart rate of ethanolic extract of papaya leaves doses of
125, 250, and 500 mg/kg bw comparable with atenolol dose of 4,5 mg/kg bw. Extract 250
mg/kg bw has the same effect with atenolol dose of 4,5 mg/kg bw in inhibiting the increase
of heart rate induced by adrenaline. Antihypertensive test with NaCl induction showed the
effect of decreasing the amplitude of the heart rate of ethanolic extract of papaya leaves
doses of 125, 250, and 500 mg/kg bw comparable with captopril dose of 13,5 mg/kg bw.
NaCl induction could not increase the heart rate so that the effect of decreasing the
frequency of the captopril and the exctract could not be observed.
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