INHIBITORY ?-GLUKOSIDASE ENZYME ACTIVITY OF ETHANOLIC EXTRACT AND ISOLATED COMPOUND FROM KATUK (SAUROPUS ANDROGYNUS (L.) MERR.)

• Main Author: Putra, Nanda
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/56558
• Institution: Institut Teknologi Bandung

Katuk (S. androgynus) has been used traditionally in India as diabetic therapy, known as “green diabetic”, but the compound that was responsible for that effect was unknown. The aim of this research were to isolate and to elucidate the structure of chemical compound which inhibit ?-glucosidase enzyme. Katuk Leaves were extracted by maceration using ethanol 70%. The extract were fractionated by liquid-liquid extraction using n-hexane, EtOAc obtained three different fractions. All of the extract and fractions were screened for ?-glucosidase inhibitory activity. The highest activity were found in EtOAc’s fraction. It was continued for further separation using vacuum liquid chromatography. FE-SA-01 (110 mg) was successfully isolated using radial chromatography. The structure determination has been done based on spectroscopic method (UV, IR and NMR/RMI). Based on the characterization’s data the isolated compound was proposed as Astragalin. The IC50 was 55.3 ± 0.61µg/mL compared to acarbose as positive control which was 162.96 ± 0.59 µg/mL. This is the first report for isolating Astragalin in Katuk Leaves.