STRUCTURAL AND HISTOLOGICAL ANALYSIS OF SILK GLAND FROM ARGIOPE APPENSA AND PLEXIPPUS PAYKULLI WITH PARAFFIN-SECTION-PROCEDURE.
Argiope appensa is an orb-weaver spider from Indonesia that produces silk. Research about silk is a growing new research which sparks interest of many scientists around the world specifically related to stem cell growth matrix. Stem cell is required for medical purposes as therapy to cure disease...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/57588 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Argiope appensa is an orb-weaver spider from Indonesia that produces silk. Research about
silk is a growing new research which sparks interest of many scientists around the world
specifically related to stem cell growth matrix. Stem cell is required for medical purposes as
therapy to cure disease for patients which appear for various products such as cartilage tissue
engineering and cartilage skin engineering. Stem cells need a nutrition medium and adjacent
matrix to support the proliferation. One of the substances of the adjacent matrix that
generally uses is Fibroin, silk from silkworm (Bombyx mori). However, the Adjacent matrix
from Fibroin is not yet optimum; hence, it is searched and found another material which one
of them is Spidroin, silk from a spider. Spidroin can be produced by the species of Argiope
appensa. The result from In-vivo takes time and the amount of the result is relatively low to
produce the spidroin. The final application of the research about spidroin is to synthesize the
spidroin not with the In-vivo method, but with the in-vitro method. In order to synthesize the
material, it is a necessity to isolate and identify the glands of silk previously. Therefore,
research and analysis about the histological structure of the silk gland of the spider needs to
be conducted using paraffin-section-procedure that will be compared with Plexippus paykulli
as a Salticidae family. Salticidae family uses their silk not to catch any prey but for
safety-line and pup-tents. In order to reveal the contrast, the section will be stained with
Hematoxylin-Eosin solution. From this research, It is found that there are three silk glands
from Argiope appensa but not in Plexippus paykulli, that is Aggregate, Aciniform, and
Pyriform, as well as two silk glands which is both of them have, that is Tubulliform and
Flagelliform.
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