STRUCTURAL AND HISTOLOGICAL ANALYSIS OF SILK GLAND FROM ARGIOPE APPENSA AND PLEXIPPUS PAYKULLI WITH PARAFFIN-SECTION-PROCEDURE.

• Main Author: Septian Anggrayana, Willy
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/57588
• Institution: Institut Teknologi Bandung

Argiope appensa is an orb-weaver spider from Indonesia that produces silk. Research about silk is a growing new research which sparks interest of many scientists around the world specifically related to stem cell growth matrix. Stem cell is required for medical purposes as therapy to cure disease for patients which appear for various products such as cartilage tissue engineering and cartilage skin engineering. Stem cells need a nutrition medium and adjacent matrix to support the proliferation. One of the substances of the adjacent matrix that generally uses is Fibroin, silk from silkworm (Bombyx mori). However, the Adjacent matrix from Fibroin is not yet optimum; hence, it is searched and found another material which one of them is Spidroin, silk from a spider. Spidroin can be produced by the species of Argiope appensa. The result from In-vivo takes time and the amount of the result is relatively low to produce the spidroin. The final application of the research about spidroin is to synthesize the spidroin not with the In-vivo method, but with the in-vitro method. In order to synthesize the material, it is a necessity to isolate and identify the glands of silk previously. Therefore, research and analysis about the histological structure of the silk gland of the spider needs to be conducted using paraffin-section-procedure that will be compared with Plexippus paykulli as a Salticidae family. Salticidae family uses their silk not to catch any prey but for safety-line and pup-tents. In order to reveal the contrast, the section will be stained with Hematoxylin-Eosin solution. From this research, It is found that there are three silk glands from Argiope appensa but not in Plexippus paykulli, that is Aggregate, Aciniform, and Pyriform, as well as two silk glands which is both of them have, that is Tubulliform and Flagelliform.