MODIFICATION OF SCREEN-PRINTED CARBON ELECTRODE (SPCE) BY THE NAFION FUNCTIONALIZED SILICON NANOPARTICLES (SINP/NAF) MATERIALS IN NON-ENZYMATIC ELECTROCHEMICAL SENSOR FOR URIC ACID (UA) DETECTION

• Main Author: Huda, Nurul
• Format: Theses
• Language: Indonesia
• Subjects: Teknik (Rekayasa, enjinering dan kegiatan berkaitan)
• Online Access: https://digilib.itb.ac.id/gdl/view/57928
• Institution: Institut Teknologi Bandung

A non-enzymatic electrochemical sensor has been successfully developed that can be used to detect uric acid (UA) based on a modified screen-printed carbon electrode (SPCE) using Nafion functionalized silicon nanoparticles. Nanoparticle silicon material is used because of its advantages, which include its abundant availability, good biocompatibility, and adjustable porosity and surface area according to the synthesis method used. Nafion is a sulfonated tetrafluoroethylene based fluoropolymer-copolymer that has been widely used for electrochemical sensors with the function of increasing the stability of the sensor to the analyte being measured. In this study, silicon material was successfully synthesized through the non-thermal chemical vapor deposition method which confirmed its structure by XRD diffractogram and morphology by SEM image. The crystallinity of the silicon material produced is 69.85%. SEM-EDS characterization was also carried out to confirm the success of the SPCE modification process by SiNP and SiNP/Naf materials. The changes in the morphology of SPCE and the EDS spectrum that were measured indicated the success of the SPCE modification process for each stage. Cyclic voltammetry, electrochemical impedance spectroscopy, and differential pulse voltammetry methods were used to analyze the electrochemical characteristics and performance of non-enzymatic electrochemical sensors based on SPCE/SiNP/Naf structures against uric acid analytes. Based on the results of electrochemical analysis, obtained sensors with sensitivity, detection limit, and quantification limit of 0.01 ?A.mM-1.cm-2, 0.21 M, and 0.69 M respectively in the linear measurement range of uric acid concentration of 10 – 1000 ?M. Then, a sensor stability study was also carried out which resulted in a relative standard deviation of 4.83%.