PENENTUAN KONSENTRASI HAMBAT MINIMUM ANTIFUNGI MELALUI PENURUNAN KADAR GLUKOSA DALAM MEDIUM PERTUMBUHAN DENGAN CARA DETEKSI KOLORIMETRI ENZIMATIK

PENENTUAN KONSENTRASI HAMBAT MINIMUM ANTIFUNGI MELALUI PENURUNAN KADAR GLUKOSA DALAM MEDIUM PERTUMBUHAN DENGAN CARA DETEKSI KOLORIMETRI ENZIMATIK

Several method for measuring minimum inhibitory concentration (MIC) from antifungal were developed due to its easyness or cost consideration, some are usually adopted for clinical use. The purpose of this research was to develop a method that uses enzymatic colorimetry for determining antifungal MIC...

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Main Author: Adetya Pertama , Errik
Format: Final Project
Language:Indonesia
Online Access:https://digilib.itb.ac.id/gdl/view/6072
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Institution: Institut Teknologi Bandung
Language: Indonesia
id id-itb.:6072
spelling id-itb.:60722012-06-18T15:03:23ZPENENTUAN KONSENTRASI HAMBAT MINIMUM ANTIFUNGI MELALUI PENURUNAN KADAR GLUKOSA DALAM MEDIUM PERTUMBUHAN DENGAN CARA DETEKSI KOLORIMETRI ENZIMATIK Adetya Pertama , Errik Indonesia Final Project INSTITUT TEKNOLOGI BANDUNG https://digilib.itb.ac.id/gdl/view/6072 Several method for measuring minimum inhibitory concentration (MIC) from antifungal were developed due to its easyness or cost consideration, some are usually adopted for clinical use. The purpose of this research was to develop a method that uses enzymatic colorimetry for determining antifungal MIC based on Trinder method using glucose oxidase kit (GOD) with sampel to reagent ratio of 1:5. Visible spectrophotometry at 550nm had been carried out to detect quinoneimin resulting from residual glucose oxidation in a liquid growth medium, Saboraud Dextrose Broth (SDB). After incubation period of 48 hours the antifungal MIC obtained from the experiment were within such stated in literature, while for 24 hours period of incubation, the MIC of amphotericin B was similar to literature MIC value. text
institution Institut Teknologi Bandung
building Institut Teknologi Bandung Library
continent Asia
country Indonesia
Indonesia
content_provider Institut Teknologi Bandung
collection Digital ITB
language Indonesia
description Several method for measuring minimum inhibitory concentration (MIC) from antifungal were developed due to its easyness or cost consideration, some are usually adopted for clinical use. The purpose of this research was to develop a method that uses enzymatic colorimetry for determining antifungal MIC based on Trinder method using glucose oxidase kit (GOD) with sampel to reagent ratio of 1:5. Visible spectrophotometry at 550nm had been carried out to detect quinoneimin resulting from residual glucose oxidation in a liquid growth medium, Saboraud Dextrose Broth (SDB). After incubation period of 48 hours the antifungal MIC obtained from the experiment were within such stated in literature, while for 24 hours period of incubation, the MIC of amphotericin B was similar to literature MIC value.
format Final Project
author Adetya Pertama , Errik
spellingShingle Adetya Pertama , Errik
PENENTUAN KONSENTRASI HAMBAT MINIMUM ANTIFUNGI MELALUI PENURUNAN KADAR GLUKOSA DALAM MEDIUM PERTUMBUHAN DENGAN CARA DETEKSI KOLORIMETRI ENZIMATIK
author_facet Adetya Pertama , Errik
author_sort Adetya Pertama , Errik
title PENENTUAN KONSENTRASI HAMBAT MINIMUM ANTIFUNGI MELALUI PENURUNAN KADAR GLUKOSA DALAM MEDIUM PERTUMBUHAN DENGAN CARA DETEKSI KOLORIMETRI ENZIMATIK
title_short PENENTUAN KONSENTRASI HAMBAT MINIMUM ANTIFUNGI MELALUI PENURUNAN KADAR GLUKOSA DALAM MEDIUM PERTUMBUHAN DENGAN CARA DETEKSI KOLORIMETRI ENZIMATIK
title_full PENENTUAN KONSENTRASI HAMBAT MINIMUM ANTIFUNGI MELALUI PENURUNAN KADAR GLUKOSA DALAM MEDIUM PERTUMBUHAN DENGAN CARA DETEKSI KOLORIMETRI ENZIMATIK
title_fullStr PENENTUAN KONSENTRASI HAMBAT MINIMUM ANTIFUNGI MELALUI PENURUNAN KADAR GLUKOSA DALAM MEDIUM PERTUMBUHAN DENGAN CARA DETEKSI KOLORIMETRI ENZIMATIK
title_full_unstemmed PENENTUAN KONSENTRASI HAMBAT MINIMUM ANTIFUNGI MELALUI PENURUNAN KADAR GLUKOSA DALAM MEDIUM PERTUMBUHAN DENGAN CARA DETEKSI KOLORIMETRI ENZIMATIK
title_sort penentuan konsentrasi hambat minimum antifungi melalui penurunan kadar glukosa dalam medium pertumbuhan dengan cara deteksi kolorimetri enzimatik
url https://digilib.itb.ac.id/gdl/view/6072
_version_ 1820663813018484736

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