PRODUKSI ENZIM SELULASE MELALUI FERMENTASI FASE PADAT PADA KULIT KACANG TANAH (ARACHIS HYPOGAEA) OLEH TRICHODERMA SP. DAN RAGI TAPE

• Main Author: Rizqullah Ramadhan, Chalil
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/61021
• Institution: Institut Teknologi Bandung

Groundnut (Arachis hypogaea) is the second most important legume commodity after soybean in Indonesia. Groundnut is one of the many food sources with very large protein content. Groundnut plant processing mostly focuses on the seeds and unfortunately leaves a lot of waste, especially the shell. Groundnut shell contains a high amount of lignocellulose and can be valorized as a substrate to produce cellulase with fungal cellulosic microbes as a biological agent. This research was conducted to determine the optimum time for cellulosic fungi to produce cellulase enzymes using groundnut shell as a substrate. In this study, solid state fermentation was used with the help of Trichoderma sp. and Aspergillus sp. as the mixed culture biological agents. Fermentation was carried at room temperature (25°C), 50% water humidity on substrates, and the ratio of substrate to culture was 10:1 with a culture ratio of 1:1. Fermentation lasts for 5 days with data sampling of cellulase activity every 24 hours. The highest enzyme activity obtained on the fourth day, with an amount of 0.12 FPU/mL. Based on the experimental enzyme activities, a mathematical modeling was carried out to determine the kinetic parameters of the cellulase enzyme production. Parameters that influence the production of cellulase are maximum specific growth rate (?m), substrate saturation constant (Ks), death cell constant (kd), maintenance coefficient (ms), yield coefficient of biomass (Yx/s), rate constant of cellulase synthesis (k1), rate constant of cellulase decay (k2), and coefficient of substrate inhibition (Ki) were determined, to 0.087; 11.3; 0.004; 0.005; 1.19; 0.004; 0.019; 1130.7, respectively.