ANALISIS TRANSKRIPTOMIK EFEK PEMATANGAN TERHADAP EKSPRESI GEN LEKTIN PADA BUAH PISANG CAVENDISH (MUSA ACUMINATA, AAA)

• Main Author: Zinedine Yuswindia, Cantika
• Format: Final Project
• Language: Indonesia
• Subjects: Ilmu hayati ; Biologi
• Online Access: https://digilib.itb.ac.id/gdl/view/61483
• Institution: Institut Teknologi Bandung

Abstract Cavendish banana is a type of climacteric fruit that is popular in the world, including in Indonesia, because of its taste and nutritional content. One of the main proteins in the ripe banana Musa acuminata is a lectin called Banana Lectin (BanLec). BanLec is a homodimeric protein which has capability to bind to mannose and has been reported to exhibit antiviral activity. Research on the expression of lectin genes in Musa acuminata bananas in unripe and ripe conditions has never been done before. A transcriptomic study was conducted to determine the effect of fruit ripening on day 7 on lectin gene expression in Cavendish banana (Musa acuminata, AAA). In this study, the results of total RNA sequencing of Cavendish bananas on day 1 and day 7 were used which were taken from the results of research by Dwivany et al. with accession number SRP227182 on NCBI. Differential gene expression (DEG) analysis of RNA-seq readings was performed using the Tuxedo protocol which consists of the TopHat, Cufflinks, Cuffmerge, Cuffdiff, and CummeRbund programs. From the results of this study, it was found that 14 lectin genes had significantly increased expression, these fourteen lectin genes were from the lectin receptor-like kinase and mannose-specific lectin groups. The lectin genes that had increased expression with the highest log2(fold change) value on day 7 were Ma08_g20280 (Putative Mannose/glucose-specific lectin (Fragment)), Ma08_g20290 (Mannose-binding lectin containing protein), and Ma08_g20240 (Putative Mannose/ glucose specific lectin (Fragment)). According to the results of gene ontology analysis, these 14 lectin genes play the most significant role in the process of phosphorylation and protein synthesis and carbohydrate binding function. According to the results of in silico analysis of protein lectin interactions with proteins involved in fruit ripening, one of the lectin proteins from the Ma10_g17960 gene (G-type lectin S-receptor-like serine/threonine-protein kinase At1g34300) was found to interact with proteins from gene Ma08_g10410 (ATP-citrate synthase beta chain protein 1-like) which plays a role in the citric acid cycle (TCA cycle). The results of this transcriptomic analysis show that the fruit ripening process for 7 days in Cavendish bananas can induce lectin gene expression, but the relationship between lectin genes and genes that play roles in fruit ripening is still not clear, so further research is needed.