KAJIAN PUSTAKA IMOBILISASI ENZIM PAPAIN DAN APLIKASINYA DALAM BIDANG FARMASI
Papain enzyme is a cysteine protease enzyme obtained from papaya latex (Carica papaya L.). The papain enzyme has less stable characteristics so it needs to be increased its stability. The process of enzyme immobilization is one way that can be done to overcome these problems. To determine the opt...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/61936 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Papain enzyme is a cysteine protease enzyme obtained from papaya latex (Carica papaya L.). The
papain enzyme has less stable characteristics so it needs to be increased its stability. The process of
enzyme immobilization is one way that can be done to overcome these problems. To determine the
optimal method and conditions during the papain enzyme immobilization process, a literature review
was carried out. There are 40 literatures obtained through searching with Science Direct and PubMed.
The results of immobilization of papain enzymes such as the amount of immobilized papain enzymes
and the results of immobilized papain enzyme activities will be influenced by several factors such as
pH, temperature, immobilization time, and additional compounds (cross-linkers). The optimum papain
enzyme immobilization was carried out at pH 6-7,5 at room temperature (25°C). The characterization
of papain enzymes such as SEM, FT-IR, BET, EDXS, XRD, VSM was carried out to determine the
immobilization of papain enzymes. The immobilized papain enzyme can maintain its activity in a range
of pH and extreme temperatures, as well as a longer storage time. In addition, the immobilized papain
enzyme is easier to obtain for repeated use. From the results of the literature review, the best method
to be used in immobilization of papain enzyme is the encapsulation method using silica. The use of
immobilized papain enzymes in the pharmaceutical field can be found in the manufacture of
hydrolysates, metal detection in pharmaceutical preparations, as wound debridement, and antibiofilm.
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