ANTITHROMBOTIC ACTIVITY OF ETHANOLIC EXTRACT OF BINAHONG LEAVES AND ITS NANOEMULSION PREPARATION (ANREDERA CORDIFOLIA) IN MALE SWISS WEBSTERâS MICE (MUS MUSCULUS)
Abnormalities of thrombus formation or known as thrombosis are disorders that underlie the occurrence of myocardial infarction and ischemic stroke, which are the cause of high mortality globally. Traditionally, people used binahong leaves as blood thinners. Therefore, the researchers aimed to obt...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/62758 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Abnormalities of thrombus formation or known as thrombosis are disorders that
underlie the occurrence of myocardial infarction and ischemic stroke, which are the
cause of high mortality globally. Traditionally, people used binahong leaves as blood
thinners. Therefore, the researchers aimed to obtain scientific data on the efficacy of
binahong as an antithrombotic.. The active compounds from binahong leaves were
extracted by maceration in ethanol solvent. The efficacy test was carried out on male
Swiss Webster strain mice with the parameters of bleeding time, coagulation time,
platelet count, and decreased plasma uptake. Three doses (50, 100, 200 mg/kgBW) of
binahong leaf extract were tested. The results showed a dose of 50 mg/kgBW had
antithrombotic activity which was indicated by an increase in bleeding time and
coagulation time 337,2 ± 30,34 (p=0,03); 181 ± 37,92 (p=0,02) after administration
of the extract for 14 days. Furthermore, the nanoemulsion preparation of binahong
leaf extract at a dose of 50 mg/kgBW was formulated using the SNEDDS (Self Nano
Emulsifying Drug Delivery System) and produced a preparation with a size of droplet
113,33 nm. Optimization of nanoemulsion preparations using virgin coconut oil as the
oil phase, tween 80 as a surfactant, and PEG 400 as a cosurfactant and produces a
stable formula. The results of the antithrombotic test showed significant antithrombotic
activity at bleeding time vs control 231,5±42,47 vs 157,17±35,02 (p=0,017),
coagulation time vs control 188±24,31 vs 171,5±22,45 (p=0,046), platelet count vs.
control 348,5±104,46 vs 463±92,51 (p=0.01), and decreased plasma uptake vs control
0,02±0,01 vs 0,08±0,04 (p=0,012). The results of the antioxidant effect test using the
DPPH method showed that the binahong leaf extract had an IC50 value of 66,08?g/mL
which was classified as a strong antioxidant. However, lipid peroxidation of binahong
leaf extract and its nanoemulsion preparations did not inhibit lipid peroxidation.
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