ANTITHROMBOTIC ACTIVITY OF ETHANOLIC EXTRACT OF BINAHONG LEAVES AND ITS NANOEMULSION PREPARATION (ANREDERA CORDIFOLIA) IN MALE SWISS WEBSTER’S MICE (MUS MUSCULUS)

• Main Author: Isna Septila, Silviyanita
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/62758
• Institution: Institut Teknologi Bandung

Abnormalities of thrombus formation or known as thrombosis are disorders that underlie the occurrence of myocardial infarction and ischemic stroke, which are the cause of high mortality globally. Traditionally, people used binahong leaves as blood thinners. Therefore, the researchers aimed to obtain scientific data on the efficacy of binahong as an antithrombotic.. The active compounds from binahong leaves were extracted by maceration in ethanol solvent. The efficacy test was carried out on male Swiss Webster strain mice with the parameters of bleeding time, coagulation time, platelet count, and decreased plasma uptake. Three doses (50, 100, 200 mg/kgBW) of binahong leaf extract were tested. The results showed a dose of 50 mg/kgBW had antithrombotic activity which was indicated by an increase in bleeding time and coagulation time 337,2 ± 30,34 (p=0,03); 181 ± 37,92 (p=0,02) after administration of the extract for 14 days. Furthermore, the nanoemulsion preparation of binahong leaf extract at a dose of 50 mg/kgBW was formulated using the SNEDDS (Self Nano Emulsifying Drug Delivery System) and produced a preparation with a size of droplet 113,33 nm. Optimization of nanoemulsion preparations using virgin coconut oil as the oil phase, tween 80 as a surfactant, and PEG 400 as a cosurfactant and produces a stable formula. The results of the antithrombotic test showed significant antithrombotic activity at bleeding time vs control 231,5±42,47 vs 157,17±35,02 (p=0,017), coagulation time vs control 188±24,31 vs 171,5±22,45 (p=0,046), platelet count vs. control 348,5±104,46 vs 463±92,51 (p=0.01), and decreased plasma uptake vs control 0,02±0,01 vs 0,08±0,04 (p=0,012). The results of the antioxidant effect test using the DPPH method showed that the binahong leaf extract had an IC50 value of 66,08?g/mL which was classified as a strong antioxidant. However, lipid peroxidation of binahong leaf extract and its nanoemulsion preparations did not inhibit lipid peroxidation.