PRODUKSI SILASE PROBIOTIK TINGGI MELALUI OPTIMASI HYDROTHERMAL PRE-TREATMENT DAN FERMENTASI LEMNA AEQUINOCTIALIS WELW. MENGGUNAKAN BAKTERI LACTOBACILLUS PLANTARUM

• Main Author: Amalia, Dara
• Format: Final Project
• Language: Indonesia
• Subjects: Teknologi
• Online Access: https://digilib.itb.ac.id/gdl/view/64190
• Institution: Institut Teknologi Bandung

Availability of feed in both quantity and quality remains a big issue for cattle farming. Duckweeds provide alternative to solve this problem due to their abundances and high nutrition value. The quality of duckweed-based feed can be improved with lactic acid fermentation using bacteria and/or by hydrothermal pre-treatment. This research aimed to produce high probiotic silage through fermentation of duckweed, Lemna aequinoctialis, using lactic acid bacteria, Lactobacillus plantarum, and by hydrothermal pre-treatment. L. aequinoctialis were hydrothermally pre-treated using autoclave for 10, 15, or 20 min. The resulted lignocellulose, starch, and glucose content were measured. Subsequently, fermentation was carried out anaerobically at 37 ? for 48 h. Glucose, bacterial cell, and lactic acid levels were measured at the end of the pre-treatment as well as lignocellulose, starch, protein and water content. The results showed that pre-treatment hydrolyses starch, cellulose, and hemicellulose into glucose. The highest glucose content (584.26 ppm) was achieved after 15 min treatment. Interestingly, hydrothermall treatment of L. aequinoctialis followed by fermentation increased the protein content in silage by 2 fold (24.81 %dw). The produced silage was also enriched with high probiotic content and lactic acid, 7.6 x 106 CFU/mL and 2,717.4 ppm, respectively. Thus, both hydrothermal pre-treatment and lactic acid fermentation could improve the quality of L. aequinoctialis silage.