SOLID STATE FERMENTATION OF MORINGA LEAF (MORINGA OLEIFERA) BY ASPERGILLUS NIGER TO INCREASE THE YIELD OF POLYPHENOL EXTRACTION USING MACERATION METHOD WITH ACETONE SOLVENT

• Main Author: Damika Putri, Adela
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/65745
• Institution: Institut Teknologi Bandung

Moringa leaves are part of the Moringa oleifera plant which is known to be rich in bioactive compounds that have the ability as antibacterial and antioxidant properties. In general, bioactive compounds are present in conjugated forms, which are linked to cell wall. Therefore, it is necessary to carry out a solid state fermentation using fungi that can produce enzymes in order to release the bonds of bioactive compounds and cell. This research was conducted to determine effect of fermentation on Moringa leaves by A. niger as a method to increase bioactivity compounds in Moringa leaf extract. Fermentation was carried out for 7 days by collecting data on lignocellulosic content and extracts obtained in 1st,3rd,5th, dan 7th day of fermentation. The highest extract yield was obtained on the 5th day of fermentation, with extract yield value of 9.71%. The results of the Moringa leaf extract were then analyzed for total flavonoid content, total phenolic content, antibacterial activity, antioxidant activity, and quantification of kaempferol content. The highest total flavonoid and phenolic content was found on the 5th day of fermentation with values of 168.34 (mg QE/g extract) and 283.20 (mg GAE/g extract). The 5th day fermented Moringa leaf extract showed antibacterial activity in E. coli, B. subtilis, and S. aureus cultures with inhibition zone diameters of 11.88 mm, 11.35 mm, and 9 mm, respectively. The concentration of kaempferol in fermented Moringa leaf extract on day 5 was 4074.32 mg/L or an increase 409% compared to unfermented Moringa leaves. In addition, statistical correlation analysis was performed to determine the effect between the extract test parameters. Based on the correlation analysis, it was found that IC50 had negative correlation on extract yields, and total phenolic content.